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抗体情報【抗原M-O】|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

抗体情報【抗原M-O】

【M】

【m】

抗原名
last update
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アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
bovine MPR2011-08-12評価者へのお問合せ 86f7WBIF IHCIP
DSHB
86f7
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
bovine MPR2011-08-12評価者へのお問合せ 22d4WBIF IHCIP
DSHB
22d4
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
bovine MPR2011-08-10評価者へのお問合せ 86f7WBIF IHCIP
DSHBx100
86f7
3%PFA (in PBS), methanol
mouse

マウス小腸
bovine MPR2011-08-10評価者へのお問合せ 22d4WBIF IHCIP
DSHBx100
22d4
3%PFA (in PBS)
mouse
マウス小腸はダメ
Not suitable for mouse small intestine.
Caco2, MDCK
c-Myc2011-08-29評価者へのお問合せ 9-E10WBIF IHCIP
Santa Cruz (BabCO)x1000x1000x500
mouse
Mono, IgG1
Lot. G050
Hela細胞
c-Myc2011-08-29評価者へのお問合せ 9-E10WBIF IHCIP
Santa Cruz (BabCO)x1000x1000x500
mouse
Mono, IgG1
Lot. G2803
Hela細胞
C-Myc2011-10-13評価者へのお問合せ WBIF IHCIP
Santa CruzX300
sc-40
mouse
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
Mono, IgG1

HeLa細胞
c-Myc(9E10)2011-10-17 WBIF IHCIP
Santa CruzX500X500
sc-40
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
CI-M6PR2011-08-29評価者へのお問合せ 2G11WBIF IHCIP
ABRx10-20
mouse
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a

Hela細胞
CI-M6PR2011-08-29評価者へのお問合せ
製品情報詳細
PolyclonalWBIF IHCIP
Abcam
ab32815
rabbit
IgG

Hela細胞
CI-M6PR (IGFIIR)2011-08-29評価者へのお問合せ WBIF IHCIP
BD Transductionx250
mouse
Mono
Rat specific, Immunogen: extracellular domain
Hela細胞
human M6PR2011-08-10評価者へのお問合せ WBIF IHCIP
GenWay Biotechx100
A21444
3%PFA (in PBS), methanol
chicken(IgY)
p

マウス小腸
human MAP LC32011-08-10評価者へのお問合せ H-50WBIF IHCIP
Santa Cruzx100
sc28266
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
human MAP LC32011-08-10評価者へのお問合せ F-14WBIF IHCIP
Santa Cruzx100
sc16756
3%PFA (in PBS), methanol
goat
p

マウス小腸
Mab4142011-10-13評価者へのお問合せ Mab414WBIF IHCIP
covanceX2000X500
MMS-120R
mouse
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
Mono, IgG1

HeLa細胞

【M】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
mAb4142014-08-01 WBIF IHCIP
COVANCEx400
MMS-120P
4%PFA/PBS
mouse
IgG1

mouse oocyte (pronucleus)
mAb4142014-08-01 WBIF IHCIP
COVANCEx400
MMS-120P
ice cold Methanol
mouse
IgG1

mouse oocyte (pronucleus)

【m】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
MACF12011-09-29評価者へのお問合せ WBIF IHCIP
AbnovaX1,000
H00023499-A01
Mouse
IF: -20℃ methanol or acetone:methanol=1:1
Poly
製品のロット間差が大きい
HeLa細胞
Mad22014-06-04評価者へのお問合せ WBIF IHCIP
Covancex500
3%PFA (in PBS)
rabbit
IgG
Make aliquots and store at -20℃. Storing at 4℃ sometimes inactivates this antibody.
HeLa
Goshima et al., JCB 2003
maltase2012-04-04評価者へのお問合せ HBB2/143/17 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG1
culture sup
Caco2
JCB 101, 838-851, 1985
Man II2011-08-29評価者へのお問合せ 53FC3WBIF IHCIP
BAbCOx1000x500
mouse
Mono, IgG2b
Ratしか使えません。
Only applicable for rat cells and tissues.
Hela細胞
MAP1A2011-08-10評価者へのお問合せ WBIF IHCIP
自家製 Homemadex100
1F4
3%PFA (in PBS)
mouse
m

マウス脳
MAP22011-08-10評価者へのお問合せ clone: HM-2WBIF IHCIP
Sigmax100
M4403
3%PFA (in PBS)
mouse
m

マウス脳
MAP22011-10-03評価者へのお問合せ
製品情報詳細
HM-2, monoclonalWBIF IHCIP
Sigma-AldrichX5000
M4403
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
MAP22012-12-12評価者へのお問合せ WBIF IHCIP
Sigmax500
M4403
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
dendrites
mouse brain
Mash12012-12-12評価者へのお問合せ WBIF IHCIP
BD pharmingenx100
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
proneural progenitors
mouse brain
Math22012-12-12評価者へのお問合せ WBIF IHCIP
Chemiconx500
AB9240
4%PFA (in PBS)
rabbit
0.1% Triton
IgG
pyramidal cell
primary hippocampal neuron
Math2 (NeuroD6)2012-12-12評価者へのお問合せ WBIF IHCIP
Santa Cruzx100
sc-19251
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
pyramidal cell
mouse brain
MDR1+32011-08-10評価者へのお問合せ clone:C219WBIF IHCIP
Zymedx100
18-7244
methanol
mouse
メタノール固定でOK
Good staining by methanol fix.
マウス肝臓
MEK1/22013-09-12 L38C12WBIF IHCIP
Cell Signaling Technologyx500
#4694
mouse
IgG1

HeLa
Mfn1 (human f.l.)2011-10-07評価者へのお問合せ 3C9WBIF IHCIP
Abnova500-1000
H00055669-M04
mouse
Mfn1, 2両方を認識するらしい
HeLa
Mfn2 (human 661-758aa)2011-10-07評価者へのお問合せ 4H8WBIF IHCIP
Abnova
H00009927-M03J
mouse
Mfn2 specific
HeLa
MHCI2011-08-29評価者へのお問合せ W6/32WBIF IHCIP
ATCC原液orx10
mouse
【PFA+Triton】:△,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono
Hybridoma Sup't、Chase後、Fixする前に必ずAcid washすること。
After chase, wash with acid before fix.
Hela細胞
MITF2011-09-17評価者へのお問合せ
製品情報詳細
MonoclonalWBIF IHCIP
Abcam
ab13703
mouse
IgG1

Hela細胞
MITF2011-09-28評価者へのお問合せ
製品情報詳細
MonoclonalWBIF IHCIP
Abcamx1000
ab13703
mouse
IgG1

HeLa細胞
MKLP12011-09-27評価者へのお問合せ WBIF IHCIP
Cytoskeleton100
AKIN06
rabbit
p
TCA, 4%PFA, 1%PFA, MeOH
HeLa
MKLP12011-09-27評価者へのお問合せ WBIF IHCIP
Santa Cruz100
sc-867
rabbit
p
TCA, 4%PFA, 1%PFA, MeOH 
HeLa
MKLP1(N-19)2011-08-29評価者へのお問合せ sc-867WBIF IHCIP
Santa Cruz x150x200-
rabbit
Poly

Hela細胞
MKLP2(Rab6KIFL, Rabkinesin6)2011-08-29評価者へのお問合せ WBIF IHCIP
BETHYL Laboratories
rabbit
Poly

Hela細胞
MKP22011-08-14評価者へのお問合せ WBIF IHCIP
BD transductionx1000
610850
mouse
m

マウス神経細胞
MLC22011-10-28評価者へのお問合せ WBIF IHCIP
Cell Signaling1/500
#3672
rabbit
m

マウス非筋細胞
MLX2011-09-17評価者へのお問合せ
製品情報詳細
PolyclonalWBIF IHCIP
Abcam
ab65147
rabbit
IgG

Hela細胞
MLX2011-09-28評価者へのお問合せ
製品情報詳細
PolyclonalWBIF IHCIP
Abcamx250
ab65147
rabbit
IgG

HeLa細胞
Moesin2011-08-29評価者へのお問合せ 38/87WBIF IHCIP
Santa Cruzx50
mouse
Mono, IgG1

Hela細胞
mouse MAP 2a,b,c Ab-32011-08-10評価者へのお問合せ WBIF IHCIP
NeoMarkersx100
MA250
3%PFA (in PBS)
mouse
m

マウス脳
mouse MAP22011-08-10評価者へのお問合せ WBIF IHCIP
自家製 Homemadex100
2G5
3%PFA (in PBS)
mouse
m

マウス脳
mouse metabtropic GluR1a2011-08-10評価者へのお問合せ WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
mouse metabtropic GluR1a2011-08-10評価者へのお問合せ WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
MRP22011-08-10評価者へのお問合せ M2?-5)=MDR2WBIF IHCIP
Alexis
ALX-801-037
mouse
m

MSH2011-08-10評価者へのお問合せ WBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACAA2201RBP84
3%PFA (in PBS)
rabbit

マウス下垂体
Myc2011-08-10評価者へのお問合せ WBIF IHCIP
Upstate
6340
rabbit

Myc2011-08-29評価者へのお問合せ WBIF IHCIP
MBLx100x100
rabbit
Poly

Hela細胞
myc2011-09-17評価者へのお問合せ #9E10WBIF IHCIP
clontechx1000
mouse
mono

Hela細胞
myc2011-09-28評価者へのお問合せ #9E10WBIF IHCIP
clontechx1000
mouse
mono

HeLa細胞
myc2011-10-03評価者へのお問合せ PL14WBIF IHCIP
MBLx1000
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Mono,IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
COS細胞,rat hippocampal neurons
myc2011-10-03評価者へのお問合せ WBIF IHCIP
MBLx1000x1000
562-5
rabbit
Poly

HEK293T細胞,COS細胞,rat hippocampal neurons
Myc-Tag2011-08-14評価者へのお問合せ 9B11WBIF IHCIP
Cell Signalingx1000x100
2276
mouse
m

【M】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
Myc-Tag2015-01-13評価者へのお問合せ WBIF IHCIP
Cell Signaling Technologyx2000
2276
3%PFA (in PBS)
Mouse
0.05%saponin
IgG2a

MEF
抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
MyHC IIB(nonmuscle)2011-09-27評価者へのお問合せ WBIF IHCIP
Covance200-500
PRB-445P
rabbit
p
4%PFA, 1%PFA, MeOH, TCA
MDCK II, EpH4, MTD-1A, DLD-1, HeLa
Myosin IIA, non muscle2011-09-27評価者へのお問合せ WBIF IHCIP
Sigma200-500
M8064
rabbit
p
4%PFA, 1%PFA, MeOH, TCA
MDCK II, EpH4, MTD-1A, DLD-1, HeLa
Myosin phospatase2011-09-27評価者へのお問合せ WBIF IHCIP
Covance100
PRB-457C
rabbit
p
1%PFA>TCA
HeLa
Myosin-B (MHC-B)2011-10-28評価者へのお問合せ WBIF IHCIP
COVANCE1/500
PRB-445P
rabbit
m

マウス非筋細胞
MyosinII a,b,c2011-08-29評価者へのお問合せ WBIF IHCIP
Plyclone

Hela細胞
MyosinVB2011-08-10評価者へのお問合せ WBIF IHCIP
LifespanBiosciencex100
LS-B2119
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
MYPT12011-10-28評価者へのお問合せ WBIF IHCIP
Cell Signaling1/1000
#2634P
rabbit
m, h

マウス非筋細胞
MYPT1 pT850 (chick) / pT853 (human)2013-02-01評価者へのお問合せ WBIF IHCIP
Upstate (Millipore)X1000
36-003
rabbit (poly)
cell lysate調製時に急速に脱リン酸化されるため、TCA処理またはSDS sample bufferによる調製を推奨 Samples should be directly prepared by TCA or SDS sample buffer because of the rapid dephosphorylation in preparation.
Nonmuscle Myosin II heavy chain2011-09-27評価者へのお問合せ WBIF IHCIP
Covance100
MMS-442P
mouse
m
蛍光染色不可
Not suitable for IF.
HeLa
p-MLC(Thr18/Ser19)2011-09-27評価者へのお問合せ WBIF IHCIP
Santa Cruz100
sc-12896
goat
p
1%PFA, 4%PFA
MDCK II
p-MYPT1 (T853)2011-10-28評価者へのお問合せ WBIF IHCIP
Cell Signaling1/1000
#4563S
rabbit
m, h
TBS使用、BSAブロッキング
Use TBS and BSA for blocking
マウス非筋細胞
Phospho myosin light chain 2 (Ser19)2011-09-27評価者へのお問合せ WBIF IHCIP
Cell Signaling100
3675S
mouse
p
1%PFA, 4%PFA
MDCK II, EpH4, MTD-1A, L
Phospho myosin light chain 2 (Thr18/Ser19)2011-09-27評価者へのお問合せ WBIF IHCIP
Cell Signaling100
3674L
rabbit
p
1%PFA, 4%PFA
MDCK II, EpH4, MTD-1A, L
Phospho-MEK1/2 (Ser217/221)2013-09-12 41G9WBIF IHCIP
Cell Signaling Technologyx1000
#9154
Rabbit monoclonal
IgG

HeLa
phospho-MLC2 (T18/S19)2011-10-28評価者へのお問合せ WBIF IHCIP
Cell Signaling1/500
#3674S
rabbit
m
TBS使用
Use TBS
マウス非筋細胞
phospho-MLC2 (T19)2011-10-28評価者へのお問合せ WBIF IHCIP
Cell Signaling1/500
#3675S
mouse
m
TBS使用
Use TBS
マウス非筋細胞
Phospho-p44/42 MAP kinase (pT202/pY204)2011-09-27評価者へのお問合せ WBIF IHCIP
Cell Signaling100
9106S
mouse
monoclonal
4%PFA
MDCK II
phosphorylated MAP1B2011-08-10評価者へのお問合せ WBIF IHCIP
自家製 Homemadex100
1B6
3%PFA (in PBS)
mouse
m

マウス脳
phosphorylated MAP1B2011-08-10評価者へのお問合せ WBIF IHCIP
自家製 Homemadex100
1B6
3%PFA (in PBS)
mouse
m

マウス脳

【n】

【N】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
beta NAP2014-04-03評価者へのお問合せ WBIF IHCIP
BD Transductionx100
610892
3%PFA (in phosphate buffer)
mouse
IgG1

mouse small intestine

【n】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
cleaved Notch12011-08-14評価者へのお問合せ WBIF IHCIP
Cell Signaling
2421S
rabbit
p

goatαNotch12011-08-10評価者へのお問合せ M-20)WBIF IHCIP
Santa Cruz
sc-6015
goat
p

Mouse CD112/Nectin-22011-09-27評価者へのお問合せ clone 502-27WBIF IHCIP
MBL100
D083-3
rat
m
1%PFA, 4%PFA, MeOH, TCA
EpH4
Mouseαrat nestin2011-08-10評価者へのお問合せ WBIF IHCIP
BD Pharmingenx100
556309
3%PFA (in PBS)
mouse
m

マウス脳
N-Cadherin2011-08-14評価者へのお問合せ WBIF IHCIP
BD transductionx100
610921
3%PFA (in PBS)
mouse
m

マウス神経細胞, MEF
Na+/K+ ATP ase2011-10-14評価者へのお問合せ C464.6WBIF IHCIP
Millipore/Upstate x 300
05-369
2% PFA 0.5%Triton, RT 15min,
methanol 固定可能
Methanol fixation also applicable
mouse
Mono、IgG1
【抗原の種、部位】rabbit whole protein
MDCK cells

【N】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
Na+K+ ATPase2015-01-13評価者へのお問合せ WBIF IHCIP
Upstate biotechnologyx100
05-369
3%PFA (in PBS)
Mouse
IgG1κ

mouse kidney frozen section
Na+K+ ATPase2015-02-27評価者へのお問合せ WBIF IHCIP
Upstate biotechnologyx100
05-369
3%PFA (in PBS)
Mouse
IgG1κ

mouse kidney frozen section
抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
Na,K-ATPase2011-11-26評価者へのお問合せ a5WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse
IgG

Drosophila nervous system
Na,K-ATPase2011-11-26評価者へのお問合せ Nrv5F7WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse
IgG

Drosophila nervous system
NaK ATPase α1subunit2012-04-04評価者へのお問合せ α6F monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx50
3%PFA in PBS
mouse
0.05% saponin
IgG2a
concentrated culture sup
Caco2
JBC 263, 4347-54, 1988
NaK ATPase αsubunit2012-04-04評価者へのお問合せ α5 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx50
3%PFA in PBS
mouse
0.05% saponin
IgG1
concentrated culture sup
Caco2
EMBO J 8, 193-202
Nectin 1 (Human)2011-09-27評価者へのお問合せ WBIF IHCIP
Santa Cruz100
sc-28639
rabbit
p
1%PFA, 4%PFA, MeOH
DLD-1
Nectin 2 (Human)2011-09-27評価者へのお問合せ WBIF IHCIP
Santa Cruz100
sc-69719
mouse
m
MeOH, 1%PFA
DLD-1
neomycin phosphotransferase II2011-08-10評価者へのお問合せ WBIF IHCIP
Upstatex1000
06747MN
rabbit
p

マウス脳
Nesprin-12011-10-13評価者へのお問合せ
製品情報詳細
PolyclonalWBIF IHCIP
AbcamX200X100
ab24742
rabbit
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
IgG

HeLa細胞
nestin2012-12-12評価者へのお問合せ WBIF IHCIP
Chemiconx500
MAB353
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
radial glial marker
mouse brain
NeuN2012-12-12評価者へのお問合せ WBIF IHCIP
Chemiconx200
MAB377
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
pan-neuron
mouse brain
NeuroD2012-12-12評価者へのお問合せ WBIF IHCIP
Santa Cruzx100
sc-1084
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
young postmitotic neuron
mouse brain
neurofilament2011-08-10評価者へのお問合せ SMI-32WBIF IHCIP
Covancex100
SMI32R
3%PFA (in PBS)
mouse
m

マウス脳
neurofilament 2002011-08-10評価者へのお問合せ NE14WBIF IHCIP
Sigamax100
N5389
3%PFA (in PBS)
mouse
m

マウス脳
Neurofilament 70k2012-12-12評価者へのお問合せ WBIF IHCIP
Milliporex200
MAB1615SP
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1

mouse brain
neurofilament,phosphorylated2011-08-10評価者へのお問合せ WBIF IHCIP
Covancex100
SMI31R
3%PFA (in PBS)
mouse

マウス脳
neuroglian2011-11-26評価者へのお問合せ BP104WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila nervous system
neuronal nuclei2011-08-10評価者へのお問合せ NeuNWBIF IHCIP
Chemiconx100
MAB377
3%PFA (in PBS)
mouse
m

マウス脳
neurons2011-11-26評価者へのお問合せ 22C10WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse
IgG

Drosophila nervous system
neurotactin2011-11-26評価者へのお問合せ BP106WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo
NGF-receptor 2011-08-10評価者へのお問合せ p75WBIF IHCIP
NeoMarkersx100
MS394
3%PFA (in PBS)
mouse
m

マウス小腸
NGF-receptor 2011-08-12評価者へのお問合せ p75WBIF IHCIP
NeoMarkers
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
NMDAR2A2011-08-10評価者へのお問合せ WBIF IHCIP
Millipore
AB1555P
3%PFA (in PBS)
rabbit
p

IFOK?
マウス脳
nNOS2013-02-01評価者へのお問合せ WBIF IHCIP
Caymanx100
160870
rabbit (poly)

Notch extra2011-11-26評価者へのお問合せ F461.3BWBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton

Drosophila embryo, discs, nervous system
Notch extra2011-11-26評価者へのお問合せ C458.2HWBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Notch intra2011-11-26評価者へのお問合せ C17.9C6WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Notch intra2011-11-26評価者へのお問合せ C17.9C6WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
NOV/CCN32012-12-12評価者へのお問合せ WBIF IHCIP
R and D systemsx500
AF1640
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
adult CA1 pyramidal cell
mouse brain
NSF2011-08-29評価者へのお問合せ WBIF IHCIP
Trunsductionx200
mouse
mono

Hela細胞
NuMA2014-06-04評価者へのお問合せ WBIF IHCIP
abcamx1000
ab36999
3% PFA (in PBS), 4%FA in PHEM
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, NCB 2012
NuMA2014-06-04評価者へのお問合せ WBIF IHCIP
cell signalingx100
3888
3% PFA (in PBS), 4%FA in PHEM
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, NCB 2012
Nup1332014-07-11 WBIF IHCIP
Proteintech Group x400
12405-1-AP
4%PFA/PBS
rabbit

mouse oocyte (pronucleus)
Nup1332014-08-01 WBIF IHCIP
Proteintech Group x400
12405-1-AP
ice cold Methanol
rabbit

mouse oocyte (pronucleus)
Nup1532016-07-11評価者へのお問合せ 7A8WBIF IHCIP
Progen1/201/10
651141
4% PFA/PBS
Mouse
IgG1

PC12
Nup352014-08-01 WBIF IHCIP
SIGMAx400
HPA018401
4%PFA/PBS
rabbit

mouse oocyte (pronucleus)
Nup352014-08-01 WBIF IHCIP
SIGMAx400
HPA018401
ice cold Methanol
rabbit

mouse oocyte (pronucleus)
Nup622012-01-19評価者へのお問合せ 53, monoclonal mouseWBIF IHCIP
BD BiosciencesX200-X500
610497
PFA, Triton
mouse
IgG2b

HeLa細胞
Nup622014-08-01 WBIF IHCIP
BD transductionx400
610497
4%PFA/PBS
mouse
IgG2b

mouse oocyte (pronucleus)
Nup622014-08-01 WBIF IHCIP
BD transductionx400
610497
ice cold Methanol
mouse
IgG2b

mouse oocyte (pronucleus)
Nup882012-01-19評価者へのお問合せ 22,monoclonalWBIF IHCIP
BD BiosciencesX300
611896
PFA, Triton
mouse
IgG1

HeLa細胞
Nup982014-08-01 WBIF IHCIP
Cell Signaling Techx400
#2598
4%PFA/PBS
rabbit

mouse oocyte (pronucleus)
Nup982014-08-01 WBIF IHCIP
Cell Signaling Techx400
#2598
ice cold Methanol
rabbit

mouse oocyte (pronucleus)
rabbit Na+/K+ ATPase a-12011-08-10評価者へのお問合せ WBIF IHCIP
Upstatex100
5369
3%PFA (in PBS)
mouse
m

マウス小腸

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評価・過去のコメント
使用細胞・組織
参考資料
mouseαOccludin2011-08-10評価者へのお問合せ WBIF IHCIP
invitrogen
33-1500
mouse
m

OCRL12011-08-29評価者へのお問合せ WBIF IHCIP
LIFESPANx100-
rabbit
【PFA+Triton】:○,【PFA+Saponin】:△,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
OCRL12011-08-29評価者へのお問合せ WBIF IHCIP
Santa Cruzダメ?ダメ
goat
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
OCRL12011-08-29評価者へのお問合せ 4A6WBIF IHCIP
Abnovax500-ダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a

Hela細胞
Oct3/42011-09-22評価者へのお問合せ WBIF IHCIP
R&D systems5 uG/ml
MAB-1759
rat
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab

mouse E3.5-6.5 embryo. TSC
Olig22012-12-11評価者へのお問合せ WBIF IHCIP
R and D systemsx1000
AF2418
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
Oligodendrocyte
mouse brain
OPA1 (human 708-830aa)2011-10-07評価者へのお問合せ 18/OPA1WBIF IHCIP
BD Transduction500-200050
No.612606
4%PFA/PBS,0.01%TritonX100
mouse

HeLa、MEF
Opsin2011-08-14評価者へのお問合せ WBIF IHCIP
SIGMA
O4886
mouse
m