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抗体情報【抗原I-L】|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

抗体情報【抗原I-L】

【I】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
Iba12012-12-11評価者へのお問合せ WBIF IHCIP
abcamx500
ab5076
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
microglia
mouse brain
IL-1 receptor2011-08-29評価者へのお問合せ 12A6WBIF IHCIP
Pharmingenx200-500
rat
Mono
mouseCD121a, TypeI/p80
Hela細胞
Insulin2011-08-10評価者へのお問合せ WBIF IHCIP
Sigmax100
I2018
3%PFA (in PBS)
mouse
m

マウス膵臓
IQGAP2011-09-27評価者へのお問合せ WBIF IHCIP
BD100
I53820
mouse
m
TCA
HeLa
IRAK2011-08-29評価者へのお問合せ 7WBIF IHCIP
BD Transductionx500x500
mouse
Mono, IgG1

Hela細胞
IκB-α2011-08-29評価者へのお問合せ C-21WBIF IHCIP
Santacruzx500x50-500
rabbit
Poly

Hela細胞
mouse I-A/I-E2011-08-10評価者へのお問合せ WBIF IHCIP
BioLegend
107601
rat

mouse IgA2011-08-10評価者へのお問合せ a chain specificWBIF IHCIP
Zymed
626700
goat

mouse IgD2011-08-10評価者へのお問合せ WBIF IHCIP
BioLegend
405701
rat

mouse IgG2011-08-10評価者へのお問合せ H+LWBIF IHCIP
Jackson
115007003
FabF goat

【J】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
JAM-A (JAM-1)2011-09-27評価者へのお問合せ WBIF IHCIP
Zymed100
36-1700
Rabbit,
polyclonal
1%PFA, MeOH
DLD-1, EpH4
JIP12011-10-03評価者へのお問合せ WBIF IHCIP
Santa Cruz×400 ×5000
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly,M-300 sc-15353

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons

【K】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
human Ki672011-08-10評価者へのお問合せ WBIF IHCIP
Novocastrax100
NCL-Ki67p
3%PFA (in PBS)
rabbit
p

マウス小腸
KAP2011-08-14評価者へのお問合せ WBIF IHCIP
BD transductionx1000x100
610334
3%PFA (in PBS)
mouse
m

マウス神経細胞
KDEL2011-08-12評価者へのお問合せ WBIF IHCIP
Stressgen
SPA827
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
KDEL2011-08-10評価者へのお問合せ WBIF IHCIP
Stressgenx100
SPA827
3%PFA (in PBS)
mouse
m

マウス膵、精巣
KDEL2011-09-17評価者へのお問合せ 10C3WBIF IHCIP
Stressgen
#SPA-827
mouse
Mono

Hela細胞
KDEL2011-09-28評価者へのお問合せ 10C3WBIF IHCIP
Stressgenx1000
#SPA-827
mouse
Mono

HeLa細胞
KDEL2011-11-26評価者へのお問合せ SPA-827WBIF IHCIP
stressgenx50
ADI-SPA-827D
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
KDEL2012-08-03評価者へのお問合せ WBIF IHCIP
Stressgenx100
SPA827
3%PFA in PBS
mouse
0.05% saponin

PC12
KDEL2013-01-19評価者へのお問合せ 10C3WBIF IHCIP
Stressgenx100
SPA827
3%PFA (in PBS)
mouse
0.05%saponin
IgG2a
弱いが、1/5くらいの細胞でそまる
Very weakly stained in -20% of all cells
Caco2細胞
KDEL receptor2011-08-14評価者へのお問合せ WBIF IHCIP
Stressgenx100
VAA-PT048
3%PFA (in PBS)
mouse
m

マウス小腸
KDEL receptor2011-11-26評価者へのお問合せ VAA-PT048WBIF IHCIP
stressgenx50
ADI-VAA-PT048D
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
KDEL-R2011-08-29評価者へのお問合せ VAA-PT048WBIF IHCIP
StressGenx200
mouse
Mono, IgG1

Hela細胞
Ki672012-12-11評価者へのお問合せ WBIF IHCIP
Novocrastax200
NCL-Ki67-MM1
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
proliferation
mouse brain
Ki672012-12-11評価者へのお問合せ WBIF IHCIP
Novocrastax200
NCL-Ki67P
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
proliferation
mouse brain
KIF13A2011-08-14評価者へのお問合せ WBIF IHCIP
Zymed
52-5407

KIF13A2011-08-10評価者へのお問合せ WBIF IHCIP
Zymedx100
525407
rabbit
p

【L】

抗原名
last update
Clone
アプリケーション   ラベルの見方はこちら
会社名希釈倍率
商品コードIF・IHC固定条件
免疫動物固定法・可溶化法
Isotype
評価・過去のコメント
使用細胞・組織
参考資料
bovine LDLR2011-08-10評価者へのお問合せ WBIF IHCIP
Progenx100
61087
3%PFA (in PBS), methanol
mouse
m

マウス小腸
FITC-Lotus2011-08-10評価者へのお問合せ WBIF IHCIP
ホーネン  J-Oil Millsx100
300219
3%PFA (in PBS)
レクチン

マウス腎臓
human lamin B2011-08-10評価者へのお問合せ C-20WBIF IHCIP
Santa Cruzx100
sc6216
3%PFA (in PBS)
goat
p

3T3, HeLa,マウス小腸、脾臓、脳など
human LAMP12011-08-10評価者へのお問合せ H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA (in PBS)
mouse

Caco2細胞
human LAMP22011-08-10評価者へのお問合せ H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA (in PBS)
mouse

Caco2細胞
human lysozyme2011-08-10評価者へのお問合せ WBIF IHCIP
DakoCytomationx100
N1515
3%PFA (in PBS)
rabbit
p
Paneth cells are stained
マウス小腸
L12011-08-10評価者へのお問合せ 8D9WBIF IHCIP
DSHBx100
8D9
3%PFA (in PBS)
mouse

マウス脳
L12011-08-10評価者へのお問合せ WBIF IHCIP
Chemiconx100
MAB5272
3%PFA (in PBS)
rat
m

マウス脳
L12011-08-12評価者へのお問合せ 8D9WBIF IHCIP
DSHB
8D9
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
L12011-08-12評価者へのお問合せ WBIF IHCIP
Chemicon
MAB5272
rat
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
L12011-10-03評価者へのお問合せ C20WBIF IHCIP
Santa cruzx1000
goat
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Lamin A+C2011-09-17評価者へのお問合せ
製品情報詳細
MonoclonalWBIF IHCIP
Abcamx1000
ab8984
mouse
IgG1

Hela細胞
Lamin A+C2011-09-28評価者へのお問合せ
製品情報詳細
MonoclonalWBIF IHCIP
Abcamx1000
ab8984
mouse
IgG1

HeLa細胞
Lamin A−Atto 647N2011-10-13評価者へのお問合せ Atto647NWBIF IHCIP
SIGMAx100
L3544
rabbit
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
Poly

HeLa細胞
lamin C (Drosophila)2011-11-26評価者へのお問合せ LC28.26WBIF IHCIP
Developmental study hybridoma bank C
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
lamin Dm0 (Drosophila)2011-11-26評価者へのお問合せ ADL84.12WBIF IHCIP
Developmental study hybridoma bank Cx25
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
lamin Dm1,2(Drosophila)2011-11-26評価者へのお問合せ ADL67.10WBIF IHCIP
Developmental study hybridoma bank Cx3000x25
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
LaminA/C2011-10-13評価者へのお問合せ XB10WBIF IHCIP
covanceX2000
MMS-107P
mouse
MeOH
Mono, IgM

HeLa細胞
LaminB2011-10-13評価者へのお問合せ 101-B7WBIF IHCIP
Matri-TectX1000X500
M1100
mouse
MeOH
Mono, IgG1

HeLa細胞
LaminB12011-10-13評価者へのお問合せ 101- B7WBIF IHCIP
CALBIOCHEMX5000X500
NA12
mouse
MeOH
Mono, IgG1

HeLa細胞
Laminin 52011-09-29評価者へのお問合せ
製品情報詳細
PolyclonalWBIF IHCIP
Abcamx1,000x500
ab14509
rabbit
IF: -20℃ methanol or acetone:methanol=1:1
IgG

HeLa細胞
LAMP12011-09-27評価者へのお問合せ H4A3WBIF IHCIP
Santa Cruz1:500~1:1000
sc-20011
mouse
m
Human細胞専用
human (HeLa, A549, MCF7)
LAMP12011-09-27評価者へのお問合せ 1D4BWBIF IHCIP
BD Biosciences1:100
553792
rat
m
mouse細胞専用
mouse (MEF, NIH3T3)
Lamp12011-10-06評価者へのお問合せ 1D4BWBIF IHCIP
eBiosciencex200
14-1071-81
Rat
4%PFA+0.1%Saponin :○
mono, IgG2a
マウスのみ
NIH3T3, マウス組織
Lamp12011-10-06評価者へのお問合せ H4A3WBIF IHCIP
eBiosciencex400
14-1079-80
mouse
4%PFA+0.1%Saponin :○
IgG1
ヒトのみ
HeLa
LAMP12011-10-13評価者へのお問合せ H4A3WBIF IHCIP
Santa CruzX200
sc-20011
mouse
Mono, IgG1

HT1080細胞
lamp12012-09-14評価者へのお問合せ H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA in PBS
mouse
0.05% saponin
IgG1
もっと薄められる
Can be diluted more
Caco2
lamp12012-09-14評価者へのお問合せ H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA in PBS
mouse
0.05% saponin
IgG1
H4A3, 1D4Bとも染まらず
Not stained by H4A3 or 1D4B Abs
MDCK
Lamp1 (CD107a)2011-08-29評価者へのお問合せ H4B3WBIF IHCIP
BD Pharmingenx5000-10000
mouse
【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1k
MDCKII ダメ、saponin使うとかなり明るい。
Not suitable for MDCKII. Good staining by saponin permeabilization.
Hela細胞
lamp22012-09-14評価者へのお問合せ H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA in PBS
mouse
0.05% saponin
IgG1
もっと薄められる
Can be diluted more
Caco2
lamp22012-09-14評価者へのお問合せ H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA in PBS
mouse
0.05% saponin
IgG1
ABL93, H4B4, GL2A7全てダメ
Not stained by ABL93, H4A4 or GL2A7 Abs
MDCK
Lamp2 (CD107b)2011-08-29評価者へのお問合せ H4B4WBIF IHCIP
BD Pharmingenx1000
mouse
【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
LAP22011-08-14評価者へのお問合せ WBIF IHCIP
BD transductionx100
611000
3%PFA (in PBS)
mouse
m

培養細胞
LAR2011-08-14評価者へのお問合せ WBIF IHCIP
BD transduction
L33420
mouse
m

LBPA2011-08-29評価者へのお問合せ 6C4WBIF IHCIP
Echelonx100
mouse
【PFA+Triton】:×,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1k

Hela細胞
LBPA(BMP)2016-12-14評価者へのお問合せ 6C4WBIF IHCIP
Echelon1/500
Z-PLBPA
4% PFA
Mouse
IgG

HeLa, PC12
LBR2011-10-13評価者へのお問合せ E398LWBIF IHCIP
EPITOMICSx100
1398-1
rabbit
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
Mono, IgG

HeLa細胞
LC32011-08-29評価者へのお問合せ WBIF IHCIP
MBLx50
rabbit
【PFA_70%EtOH】:○【PFA条件】3%PFA/PBS 37℃10min
Poly

Hela細胞
LC32011-09-27評価者へのお問合せ WBIF IHCIP
MBL1:10001:500~1:1000
PM036
rabbit
p

MEF ,NIH3T3, HeLa, A549, MCF7他
LC3B2011-08-10評価者へのお問合せ WBIF IHCIP
sigma
L7543
rabbit
p

LDLR2011-08-29評価者へのお問合せ C7WBIF IHCIP
ATCCダメ(?)x100
mouse
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
mono
Hybridoma Supernatant
Hela細胞
Lefty-12011-09-22評価者へのお問合せ WBIF IHCIP
R&D systems4 ug/ml
MAB9941
rat
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab IgG2A

mouse E6.5 embryo
LGN2014-06-04評価者へのお問合せ WBIF IHCIP
Bethyl Laboratoriesx1000
A303-032A
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, Cell 2013
luciferase2011-09-17評価者へのお問合せ WBIF IHCIP
Promega
#G745A
goat
Poly

Hela細胞
luciferase2011-09-28評価者へのお問合せ WBIF IHCIP
Promegax1000
#G745A
goat
Poly

HeLa細胞
mouse LAMP12011-08-10評価者へのお問合せ 1D4BWBIF IHCIP
DSHBx100
1D4B
3%PFA (in PBS)
rat
ImEMにも使用可能
Applicable for ImEM
マウス小腸
mouse LAMP22011-08-12評価者へのお問合せ WBIF IHCIP
DSHB
ABL93
rat
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
mouse LAMP22011-08-10評価者へのお問合せ GL2A7WBIF IHCIP
DSHB
GL2A7
rat

mouse LAMP22011-08-10評価者へのお問合せ ABL93WBIF IHCIP
DSHBx100
ABL93
3%PFA (in PBS)
rat
ImEMには使えない(GAで失活)が、PFAでは一番よい
Not suitable for ImEM because antigenicity is lost by GA. Stains LAMP2 best of all lamp2 Abs by PFA fix.
マウス小腸
mouse LDLR2011-08-10評価者へのお問合せ NS0WBIF IHCIP
R&D Systemsx1000x100
AF2255
3%PFA (in PBS)
goat

マウス小腸、腎臓
mouse LDLR2011-08-10評価者へのお問合せ M-20WBIF IHCIP
Santa Cruzx100
sc11826
3%PFA (in PBS), methanol
goat
p

マウス小腸
mouse Ly-6G/Ly-6C2011-08-10評価者へのお問合せ Gr-1WBIF IHCIP
BioLegend
108401
rat

ovine LHb2011-08-10評価者へのお問合せ WBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACOV27b01RBP85
3%PFA (in PBS)
rabbit

マウス下垂体