日本細胞生物学会
HOME- Home
- >
- Experimental protocol top
- >
- Immunofluorescence of tissue sections（5〜10μm）
Immunofluorescence of tissue sections（5〜10μm）
＊download PDF| Author | Akihiro Harada | ||
|---|---|---|---|
| Affiliation | Department of Cell Biology, Graduate School of Medicine, Osaka University | ||
| Contact | inquiry about this protocol | ||
| Home Page | http://www.med.osaka-u.ac.jp/pub/acb/eng.html | ||
| Keyword | |||
| Published | 2015-08-26 | Last Update | 2015-08-26 |
| View | 9 | download PDF | 3 |
Summary
Methods
Detail ＊Click photo to see a large size.
- Washi with PBS 5min×3（to remove OCT compound）
- 5％Normal donkey serum in PBS 5-10min
- diluted primary antibody 37℃1hr
- Wash with PBS 5min×3
- 5％Normal donkey serum in PBS 5-10min
- diluted secondary antibody RT1hr
- Wash with PBS, 5min×3
- embedding
A put a drop of mounting medium (or PBS) onto the section on slide and cover the section with a coverslip.
B seal the coverslip with nail polish 5％Normal donkey serum in PBS Normal donkey serum(CHEMICON S30-100ML) Aliquot and store at -20℃. Dilute with PBS to 5％ on usage.
