日本細胞生物学会Japan Society for Cell Biology

Cryoprotection

AuthorMasataka Kunii
AffiliationDepartment of Cell Biology, Graduate School of Medicine, Osaka University
Contactinquiry about this protocol
Home Pagehttp://www.med.osaka-u.ac.jp/pub/acb/eng.html
Keyword
Published2015-08-26Last Update2015-08-26
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Summary

Methods

Detail  *Click photo to see a large size.

    • transfer samples from the fixative to 4%sucrose-0.1M phosphate buffer (PB), 4℃, 15min×2
    • transfer to 10%sucrose-0.1M PB, 30min (until the samples sink to the bottom of the vial)
    • transfer to 15%sucrose-0.1M PB, 30min (the same as above)
    • transfer to 20%sucrose-0.1M PB, 30min(O/N)
    • transfer to 2.3M sucrose or 20%PVP-1.84M sucrose (only for cryoimmunoEM)

Little trick

Reference