Taking out brain samples
|Affiliation||Department of Cell Biology, Graduate School of Medicine, Osaka University|
|Contact||inquiry about this protocol|
Detail ＊Click photo to see a large size.
- cut the bone between eyes with a large scissors and then cut the middle of the skull to the most rostral part of cerebellum. Then cut the bone along the rostral edges (right and left edges) of cerebellum. Remove bone pieces with tweezers and expose cerebrum and cerebellum. Insert the blade of a metal earpick under the brain to cut the cranial nerve by turning over the brain caudally. If necessary, cut the medulla oblongata or spinal cord to take out the brain.
- separate the cerebrum and cerebellum by cutting the brain by their junction with pieces of razor blades
- Sampling cerebellum: take out the vermis of the cerebellum by cutting the both sides of vermis. hippocampus: Remove the brain stem by inserting a blade of a metal earpick into the border between brain stem and the cortex, and then cut the brain stem by the blade. After exposing the hippocampus, isolate it with pieces of razor blade. After getting the whole hippocampus, slice the hippocampus perpendicular to the long axis (like making banana slices).
- fix the samples in the vials with fixative for 2hr RT.