Antibody（cytoskeleton）｜一般社団法人　日本細胞生物学会

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Antibody（cytoskeleton）

Antibody（cytoskeleton）

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Actin2011-08-29	C4	WB	IF	IHC	IP
	CHEMICON	x2000

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1k
	Hela細胞
a-actinin-42011-08-10	N-term	WB	IF	IHC	IP
	Zymed		x100
	421400	3%PFA (in PBS)
	rabbit
	p

AKAP4502018-08-09	Clone 7	WB	IF	IHC	IP
	BD Biosciences		1/500
	611518	MeOH
	Mouse

	RPE1, HeLa, U2OS
l-Afadin2011-09-27		WB	IF	IHC	IP
	Sigma		200
	A0349
	rabbit
	p	1%PFA, 4%PFA, MeOH, TCA
	MDCK II, MTD-1A, DLD-1,その他多くのマウス組織
β-Actin2011-09-27	clone AC-15	WB	IF	IHC	IP
	Sigma		100
	A1978
	mouse
	m	MeOH, TCA
	HeLa
α-Actinin2011-09-27	BM-75.2	WB	IF	IHC	IP
	Sigma		100
	A5044
	mouse
	m	不明瞭
	MDCK II
Actin (20-33)2011-09-27	clone 20-33	WB	IF	IHC	IP
	Sigma		100
	A5060
	rabbit
	p	4%PFA, MeOH, TCA

Actin (20-33)2011-10-13		WB	IF	IHC	IP
	SIGMA	x1000~
	A5060
	rabbit
	Poly
	TIG-1細胞
β-Actin2011-09-27	clone AC-74	WB	IF	IHC	IP
	Sigma		100
	A5316
	mouse
	m	MeOH, TCA
	HeLa
b-actin2011-10-04	AC-15	WB	IF	IHC	IP
	Sigma	X2000
	A5441
	mouse
	Mono,IgG1
	HL-60細胞
β-Actin2011-10-14	AC-15	WB	IF	IHC	IP
	Sigma		x10,000
	A5441	methanol 固定可能 methanol fixation also applicable
	mouse
	Mono、IgG1	【抗原の種、部位】N-terminal peptide
	MDCK cells, Hela-K cells
b-actin2011-08-10 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam	x1000
	ab6276
	mouse
	IgG1
	マウス
Alpha-actinin-42011-09-27		WB	IF	IHC	IP
	Alexis		100
	ALX-210-356- C050
	Rabbit
	p	TCA
	EpH4
a-actinin-42011-08-10		WB	IF	IHC	IP
	Alexis		x100
	ALX210356	3%PFA (in PBS)
	rabbit
	p
	マウス小腸
actin2011-10-03	C4	WB	IF	IHC	IP
	Chemicon	x1000?3000
	MAB1501R
	mouse
	Mono, IgG1k
	HEK293T細胞、rat hippocampal neurons
ASPM2018-07-12		WB	IF	IHC	IP
	Novus Biologicals		1/250
	NB100-2278	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
α-tubulin2018-07-12		WB	IF	IHC	IP
	MBL		1/500
	PM054	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Alpha-actinin2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-17829
	mouse
	m	MeOH, TCAでストレスファイバー弱く染める Stress fibers are stained by MeOH or TCA fix.
	HeLa
A cyclase?2011-08-10		WB	IF	IHC	IP
	Santa Cruz
	sc-588
	rabbit
	p	染めている人からの情報 Information from other users 繊毛が染まるはず Expected to stain cilia

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
α-Tubulin2017-09-14	B-5-1-2	WB	IF	IHC	IP
	Sigma	1/2000
	T 6074
	Mouse
	IgG1
	zebrafish embryos (8-10 hpf/hours post fertilization)

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
α-tubulin2018-07-12		WB	IF	IHC	IP
	Sigma		1/1000
	T5168	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【C】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
CLASP22011-09-29	KT69	WB	IF	IHC	IP
	absea		x200-500
	032012H02
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG2b
	HeLa細胞
Cortactin2011-10-03	4F11	WB	IF	IHC	IP
	Millipore	x1000	x100?500
	05-180
	mouse	【Formalin in PBS+0.05%Triton】：○, 【Formalin in Ringer液+0.05%Triton】：○, 【Formalin in PBS+Methanol】：○, 【Formalin in Ringer液+Methanol】：○,
	Mono,IgG1	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
CPAP/CENP-J2018-07-12		WB	IF	IHC	IP
	Proteintech		1/100
	11517-1-AP	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
CP1102018-07-12		WB	IF	IHC	IP
	proteintech		1/1000
	12780-1-AP	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa

【c】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Phospho-cofillin2015-01-13		WB	IF	IHC	IP
	Cell Signaling Technology	x1000
	3313	3%PFA (in PBS)
	Rabbit	0.05%saponin
	IgG
	MEF
Cofillin2015-01-13		WB	IF	IHC	IP
	Cell Signaling Technology	x500
	5175	3%PFA (in PBS)
	rabbit	0.05%saponin
	IgG
	MEF

【C】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human active-b-catenin2011-08-10	ABC 8E7	WB	IF	IHC	IP
	Upstate	x1000	x100
	5665	3%PFA (in PBS)
	mouse
	m
	マウス小腸
Cep1922018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A302-324A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Cep1522018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A302-480A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
CEP1282018-07-26		WB	IF	IHC	IP
	abcam		1/500
	ab118797	MeOH
	Rabbit

	RPE1, HeLa, U2OS
Cytokeratin, pan2011-09-27	clone PCK-26	WB	IF	IHC	IP
	Sigma		100
	C1801
	mouse
	m	4%PFA>MeOH, TCA
	A431
α-Catenin2011-09-27		WB	IF	IHC	IP
	Sigma		200
	C2081
	rabbit
	p	1%PFA, 4%PFA, MeOH, TCA
	HeLa, MDCK II, EpH4, DLD-1, NRK,多くのマウス組織
β-Catenin2011-08-10		WB	IF	IHC	IP
	Sigma	x1000	x100
	C2206	3%PFA (in PBS)
	rabbit

	マウス小腸
Cytokeratin, pan2011-09-27	clone C-11	WB	IF	IHC	IP
	Sigma		100
	C2931
	mouse
	m	MeOH, TCA
	MDCK II
CDK5RAP22018-07-12		WB	IF	IHC	IP
	Bethyl laboratories		1/500
	IHC00063	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
CLIP1702011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-12799
	goat
	p	不明瞭 Staining not evident
	HeLa
Clasp12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-33476
	goat
	p	MeOH
	HeLa

【D】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Dynein heavy chain2011-08-29		WB	IF	IHC	IP
	トランスジェニック Trans Genic Inc.

	rabbit
	Poly
	Hela細胞
Dynein intermediate chain12011-09-29 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam	x1,000
	ab23905
	mouse
	IgG2b

Dynein intermediate chain2011-08-29	70/1	WB	IF	IHC	IP
	Sigma
	D5167
	mouse
	Mono
	Hela細胞
Dynein (Intermediate Chain)2011-09-29	70.1	WB	IF	IHC	IP
	sigma	x1,000
	D5167
	mouse
	mono

Dynein HC2011-09-29		WB	IF	IHC	IP
	Santa Cruz	x200~500
	sc-9115
	rabbit

【e】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
enabled (drosophila)2016-08-10	5G2	WB	IF	IHC	IP
	Developmental Studies Hybridoma Bank (DSHB)		x100
		4%PFA (in phosphate buffer)
	Mouse	0.1% TritonX-100/PBS
	IgG1
	Drosohila imaginal disc

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
EB32011-09-29	KT36	WB	IF	IHC	IP
	absea		x200
	010314H04
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG2a
	HeLa細胞
EB22011-09-29	KT52	WB	IF	IHC	IP
	absea		x200
	010614A11
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG2b
	HeLa細胞
EB1&32012-08-29	KT65	WB	IF	IHC	IP
	absea		x10
	010815H11S
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG2a
	HeLa細胞
Ezrin2011-09-27	EP886Y	WB	IF	IHC	IP
	EPT		100
	1729-1

		TCA, 4%PFA
	HeLa
EB12011-09-29	5	WB	IF	IHC	IP
	BD Transduction Lab.		x200
	610535
	mouse	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG1
	HeLa細胞
Eplin2011-09-27		WB	IF	IHC	IP
	BD		100
	612114
	mouse
	m	1%PFA, TCA
	DLD-1
EB2 / MAPRE22012-08-23		WB	IF	IHC	IP
	abcam	x500
	ab123579	IF: -20℃ methanol or acetone:methanol=1:1
	rabbit

	HeLa細胞
EB3 / MAPRE32012-08-23		WB	IF	IHC	IP
	abcam	x1,000-2,000	x500-1,000
	ab126953	IF: -20℃ methanol or acetone:methanol=1:1
	rabbit

	HeLa細胞
Phospho-Ezrin2011-09-27		WB	IF	IHC	IP
	Chemicon		100
	AB3832
	rabbit
	p	TCA>>4%PFA Ezrinに特異性はなくRadixin, Moesinも同様に認識。 Stains ezrin, radixin, and moesin.
	HeLa
EB1/MAPRE12012-08-23 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	x1,000-5,000	x200-1,000
	ab50188
	rabbit	IF: -20℃ methanol or acetone:methanol=1:1
	IgG	チューブリンにも反応するので要注意 Also recognize tubulin
	HeLa細胞
EB32012-08-29 About Product	KT36	WB	IF	IHC	IP
	abcam		x200
	ab53360
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	IgG2a
	HeLa細胞
Ezrin2011-10-14	3C12	WB	IF	IHC	IP
	SIGMA	x 1000	x 500
	E8897	2％ PFA 0.5%Triton, RT 15min
	mouse
	Mono、IgG1	【抗原の種、部位】human 362-585 a.a.
	MDCK cells
EB12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-15347
	rabbit
	p	MeOH
	HeLa
EB12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-47704
	mouse
	m	MeOH>TCA
	HeLa
Ezrin2011-08-10	3C12	WB	IF	IHC	IP
	Santa Cruz
	sc-58758
	mouse
	m

【F】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Fascin2011-09-27		WB	IF	IHC	IP
	Chemicon		100
	MAB3582
	mouse
	m	TCA>MeOH>1%PFA
	HeLa

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GM1302018-08-09		WB	IF	IHC	IP
	Cell Signaling	1/3000	1/5000
	12480	MeOH, PFA
	Rabbit	0.05% triton X-100 for PFA fixation

	RPE1, HeLa, U2OS
γ-tubulin2018-07-26	TU-30	WB	IF	IHC	IP
	abcam		1/500
	ab27074	MeOH
	Mouse

	RPE1, HeLa, U2OS
γ-tubulin2018-07-12	GTU88	WB	IF	IHC	IP
	Sigma		1/1000
	T6557	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【H】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
HKLP２2011-09-27		WB	IF	IHC	IP
	Cytoskeleton		100
	AKIN13
	rabbit
	p	TCA>MeOH>1%PFA
	HeLa

【I】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
IQGAP2011-09-27		WB	IF	IHC	IP
	BD		100
	I53820
	mouse
	m	TCA
	HeLa

【J】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
JIP12011-10-03		WB	IF	IHC	IP
	Santa Cruz	×400	×5000

	rabbit	【Formalin in PBS+0.05%Triton】:○
	Poly,M-300 sc-15353	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons

【K】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
KIF13A2011-08-14		WB	IF	IHC	IP
	Zymed
	52-5407



KIF13A2011-08-10		WB	IF	IHC	IP
	Zymed		x100
	525407
	rabbit
	p

Katanin p60 AL12018-07-26	A-10	WB	IF	IHC	IP
	Santa Cruz Biotechnology		1/100
	sc-373814	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa

【L】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
LBR2011-10-13	E398L	WB	IF	IHC	IP
	EPITOMICS		x100
	1398-1
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
	Mono, IgG
	HeLa細胞
Lamin A−Atto 647N2011-10-13	Atto647N	WB	IF	IHC	IP
	SIGMA		x100
	L3544
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
	Poly
	HeLa細胞
LaminB2011-10-13	101-B7	WB	IF	IHC	IP
	Matri-Tect	X1000	X500
	M1100
	mouse	MeOH
	Mono, IgG1
	HeLa細胞
LaminA/C2011-10-13	XB10	WB	IF	IHC	IP
	covance	X2000
	MMS-107P
	mouse	MeOH
	Mono, IgM
	HeLa細胞
LaminB12011-10-13	101- B7	WB	IF	IHC	IP
	CALBIOCHEM	X5000	X500
	NA12
	mouse	MeOH
	Mono, IgG1
	HeLa細胞
human lamin B2011-08-10	C-20	WB	IF	IHC	IP
	Santa Cruz		x100
	sc6216	3%PFA (in PBS)
	goat
	p
	3T3, HeLa,マウス小腸、脾臓、脳など

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Moesin2011-08-29	38/87	WB	IF	IHC	IP
	Santa Cruz		x50

	mouse
	Mono, IgG1
	Hela細胞
MKLP2(Rab6KIFL, Rabkinesin6)2011-08-29		WB	IF	IHC	IP
	BETHYL Laboratories

	rabbit
	Poly
	Hela細胞
MKLP1(N-19)2011-08-29	sc-867	WB	IF	IHC	IP
	Santa Cruz	x150	x200-

	rabbit
	Poly
	Hela細胞
MyosinII a,b,c2011-08-29		WB	IF	IHC	IP
	Plyclone



	Hela細胞
MYPT12011-10-28		WB	IF	IHC	IP
	Cell Signaling	1/1000
	#2634P
	rabbit
	m, h
	マウス非筋細胞
MLC22011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3672
	rabbit
	m
	マウス非筋細胞
phospho-MLC2 (T18/S19)2011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3674S
	rabbit
	m	TBS使用 Use TBS
	マウス非筋細胞
phospho-MLC2 (T19)2011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3675S
	mouse
	m	TBS使用 Use TBS
	マウス非筋細胞
p-MYPT1 (T853)2011-10-28		WB	IF	IHC	IP
	Cell Signaling	1/1000
	#4563S
	rabbit
	m, h	TBS使用、BSAブロッキング Use TBS and BSA for blocking
	マウス非筋細胞
phosphorylated MAP1B2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1B6	3%PFA (in PBS)
	mouse
	m
	マウス脳
phosphorylated MAP1B2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1B6	3%PFA (in PBS)
	mouse
	m
	マウス脳
MAP1A2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1F4	3%PFA (in PBS)
	mouse
	m
	マウス脳
mouse MAP22011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	2G5	3%PFA (in PBS)
	mouse
	m
	マウス脳
MYPT1 pT850 (chick) / pT853 (human)2013-02-01		WB	IF	IHC	IP
	Upstate (Millipore)	X1000
	36-003
	rabbit (poly)
		cell lysate調製時に急速に脱リン酸化されるため、TCA処理またはSDS sample bufferによる調製を推奨 Samples should be directly prepared by TCA or SDS sample buffer because of the rapid dephosphorylation in preparation.

Phospho myosin light chain 2 (Thr18/Ser19)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	3674L
	rabbit
	p	1%PFA, 4%PFA
	MDCK II, EpH4, MTD-1A, L
Phospho myosin light chain 2 (Ser19)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	3675S
	mouse
	p	1%PFA, 4%PFA
	MDCK II, EpH4, MTD-1A, L
MKLP12011-09-27		WB	IF	IHC	IP
	Cytoskeleton		100
	AKIN06
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa
MACF12011-09-29		WB	IF	IHC	IP
	Abnova		X1,000
	H00023499-A01
	Mouse	IF: -20℃ methanol or acetone:methanol=1:1
	Poly	製品のロット間差が大きい
	HeLa細胞
MyosinVB2011-08-10		WB	IF	IHC	IP
	LifespanBioscience		x100
	LS-B2119	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
MAP22011-08-10	clone: HM-2	WB	IF	IHC	IP
	Sigma		x100
	M4403	3%PFA (in PBS)
	mouse
	m
	マウス脳
MAP22011-10-03 About Product	HM-2, monoclonal	WB	IF	IHC	IP
	Sigma-Aldrich		X5000
	M4403
	mouse	【Formalin in PBS+0.05%Triton】:○, 【Formalin in PBS+Methanol】:○,
		ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
Myosin IIA, non muscle2011-09-27		WB	IF	IHC	IP
	Sigma		200-500
	M8064
	rabbit
	p	4%PFA, 1%PFA, MeOH, TCA
	MDCK II, EpH4, MTD-1A, DLD-1, HeLa
mouse MAP 2a,b,c Ab-32011-08-10		WB	IF	IHC	IP
	NeoMarkers		x100
	MA250	3%PFA (in PBS)
	mouse
	m
	マウス脳
Nonmuscle Myosin II heavy chain2011-09-27		WB	IF	IHC	IP
	Covance		100
	MMS-442P
	mouse
	m	蛍光染色不可 Not suitable for IF.
	HeLa
MyHC IIB(nonmuscle)2011-09-27		WB	IF	IHC	IP
	Covance		200-500
	PRB-445P
	rabbit
	p	4%PFA, 1%PFA, MeOH, TCA
	MDCK II, EpH4, MTD-1A, DLD-1, HeLa
Myosin-B (MHC-B)2011-10-28		WB	IF	IHC	IP
	COVANCE		1/500
	PRB-445P
	rabbit
	m
	マウス非筋細胞
Myosin phospatase2011-09-27		WB	IF	IHC	IP
	Covance		100
	PRB-457C
	rabbit
	p	1%PFA>TCA
	HeLa
p-MLC(Thr18/Ser19)2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-12896
	goat
	p	1%PFA, 4%PFA
	MDCK II
MKLP12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-867
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
NuMA2018-07-26		WB	IF	IHC	IP
	Cell Signaling Technology		1/1000
	3888S	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Mouseαrat　nestin2011-08-10		WB	IF	IHC	IP
	BD Pharmingen		x100
	556309	3%PFA (in PBS)
	mouse
	m
	マウス脳
Nesprin-12011-10-13 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	X200	X100
	ab24742
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	IgG
	HeLa細胞
NEDD12018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab57336	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
neurofilament 2002011-08-10	NE14	WB	IF	IHC	IP
	Sigama		x100
	N5389	3%PFA (in PBS)
	mouse
	m
	マウス脳
NDC802018-07-26	C-11	WB	IF	IHC	IP
	Santa Cruz Biotechnology		1/50
	sc-515550	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
neurofilament,phosphorylated2011-08-10		WB	IF	IHC	IP
	Covance		x100
	SMI31R	3%PFA (in PBS)
	mouse

	マウス脳
neurofilament2011-08-10	SMI-32	WB	IF	IHC	IP
	Covance		x100
	SMI32R	3%PFA (in PBS)
	mouse
	m
	マウス脳

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Peanut2011-11-26	4C9H4	WB	IF	IHC	IP
	Developmental study hybridoma bank S
		4％PFA +0.1％Triton
	mouse
	IgG
	Drosophila embryo, discs, nervous system
p150Glued2011-09-29	12	WB	IF	IHC	IP
	BD Transduction Lab.		x200
	612709
	mouse	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG1
	HeLa細胞
Plk12018-07-26		WB	IF	IHC	IP
	Bethyl laboratories		1/1000
	A300?251A	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab28144	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/2000
	ab4448	MeOH, PFA
	Rabbit	0.05% triton X-100 for PFA fixation

	RPE1, HeLa, U2OS
pericentrin2018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab4448	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Polyglutamylation Modification2018-07-26	GT335	WB	IF	IHC	IP
	AdipoGen		1/1000
	AG-20B-0020-C100	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Polyglutamylated tubulin2018-08-09	GT335	WB	IF	IHC	IP
	AdipoGen		1/500
	AG-20B-0020-C100	MeOH
	Mouse

	RPE1, HeLa, U2OS
Plk42018-07-26	6H5	WB	IF	IHC	IP
	Merck Millipore		1/500
	MABC544	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
p150 Glued2011-08-14		WB	IF	IHC	IP
	BD transduction
	P41920
	mouse
	m

Pericentrin2011-09-27		WB	IF	IHC	IP
	Covance		100
	PRB-432C
	rabbit
	p	MeOH, TCA
	MDCK II
Rabbit polyPericentrin co.VANCE2011-08-10		WB	IF	IHC	IP
	COVANCE		x100
	prb432c	3%PFA (in PBS)
	rabbit
	p
	マウス胎児脳
human Pin12011-08-10	H-123	WB	IF	IHC	IP
	Santa Cruz
	sc15340
	rabbit
	p	未使用 Not used so far

【R】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
ROCK12013-02-01	EPR638Y	WB	IF	IHC	IP
	Epitomics	x500	x200
	1953-1	4% PFA
	rabbit (mono)

	HeLa

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
spectrin2011-11-26	3A9	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
human CDCrel-12011-08-10	septin 5	WB	IF	IHC	IP
	IBL		x100
	18921	3%PFA (in PBS), methanol
	rabbit

	マウス小腸
human septin 72011-08-10	C	WB	IF	IHC	IP
	IBL		x100
	18991	3%PFA (in PBS), methanol
	rabbit

	マウス小腸
SEPT62011-08-14		WB	IF	IHC	IP
	Atlas		x100
	HPA005665	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
Scrib2013-02-01	7C6.D10	WB	IF	IHC	IP
	Millipore		x100
	MAB1820	3.7% FA
	mouse
	IgG1
	MDCK2

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
synaptophysin/p382014-05-09		WB	IF	IHC	IP
	Millipore (Roche)		x1000
	MAB5258	3%PFA (in phosphate buffer)
	mouse
		pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
	mouse cerebrum, cerebellum

【T】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
b-Tubulin2011-08-29	KMX-1	WB	IF	IHC	IP
	Chemicon		x1000

	mouse	【PFA+Triton】:○,【TCA】:○【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG2b
	Hela細胞
AcetylTubulin2011-08-29		WB	IF	IHC	IP
	SIGMA		x200-

	mouse	【PFA+Triton】:◎,◎(CanGetSignalA (from TOYOBO Inc.)),【TCA】:◎,◎(CanGetSignalA (from TOYOBO Inc.))【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Mono、IgG2b
	Hela細胞
tubulin(β)2011-11-26		WB	IF	IHC	IP
	Developmental study hybridoma bank	x100

	mouse

	Drosophila embryo, discs, nervous system
α-tubulin2011-10-03		WB	IF	IHC	IP
	Cell Signaling		×250
	2144
	rabbit	【Formalin in PBS+0.05%Triton】:○
	Poly	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
Glu-alpha-tublin2011-09-27		WB	IF	IHC	IP
	SS		100
	302 011
	mouse
	m	不明瞭 Staining not evident
	HeLa
Tyr-alpha-tub2011-09-27		WB	IF	IHC	IP
	SS		100
	302-111
	mouse
	m	不明瞭 Staining not evident
	HeLa
Detyrosinated (Glu-)tublin2011-09-27		WB	IF	IHC	IP
	chemicon		100
	AB3201
	rabbit
	p	不明瞭 Staining not evident
	HeLa
Tubulin, alpha2011-08-29 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam
	ab6160
	rat	【TCA】:○【TCA条件】TCA 10%/H2O on ice 15min
	IgG2a	凍るためGlycerol添加（final 66.6%、a-Tubulin 50ul + glycerol 25 ul） To prevent freezing, add glycerol （final 66.6%、a-Tubulin 50ul + glycerol 25 ul）
	Hela細胞
Tubulin, alpha2011-09-29 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam	x5,000~	x50~100
	ab6160
	rat	IF: -20℃ methanol or acetone:methanol=1:1
	IgG2a
	HeLa細胞
tubulin2011-10-28		WB	IF	IHC	IP
	Cytoskeleton		x400
	ATN02	4%PFA+0.1%Saponin :○
	Sheep
	polyclonal
	HeLa
α-Tubulin−FITC2011-09-29	DM1A	WB	IF	IHC	IP
	SIGMA	-	x50~100
	F2168
	mouse	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG1
	HeLa細胞
Tau12011-08-14		WB	IF	IHC	IP
	CHEMICON	x1000	x100
	MAB3420	3%PFA (in PBS)
	mouse
	m
	マウス脳
bovine tau-12011-08-10		WB	IF	IHC	IP
	Chemicon		x100
	MAB3420	3%PFA (in PBS)
	mouse
	m
	マウス脳
Tau-12011-10-03	PC1C6	WB	IF	IHC	IP
	Chemicon		x500
	MAB3420
	mouse	【Formalin in PBS+0.05%Triton】:○, 【Formalin in PBS+Methanol】:○,
	Mono, IgG2a	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
mouseαNeuronal classαTubulin2011-08-10		WB	IF	IHC	IP
	Covance		x100
	mms-435p	3%PFA (in PBS)
	mouse
	m
	マウス脳
beta 3-tubulin2011-10-03	Tuj1	WB	IF	IHC	IP
	Covance		X2000
	MMS-435P
	mouse	【Formalin in PBS+0.05%Triton】:○, 【Formalin in PBS+Methanol】:○,
	Mono, IgG2a	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
alpha-tubulin2018-07-12		WB	IF	IHC	IP
	MBL	1/2000
	PM054
	rabbit

	Drosophila pupa
Tubulin2011-09-27		WB	IF	IHC	IP
	Sigma		100
	T3526
	rabbit
	p	染まらず Not stained
	MDCK II
β-Tubulin2011-08-10	clone:TUB2.1	WB	IF	IHC	IP
	Sigma		x100
	T4026	3%PFA (in PBS)
	mouse
	m
	マウス脳など
γ-Tubulin2011-08-10		WB	IF	IHC	IP
	SIGMA		x100
	T5192	3%PFA (in PBS), methanol
	rabbit
	p	メタノール固定でＯＫ Methanol fixation alone is also suitable.
	マウスＭＥＦ
γ-Tubulin2011-10-17		WB	IF	IHC	IP
	Sigma		X500
	T5192
	rabbit	【MeOH -20℃ 5min】:○ 【3.7％ formalin solution 37℃ 5min+MeOH -20℃ 15min】:○, 【3.7％ formalin solution+Triton】:×
	Poly	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	HeLa細胞
tubulin2011-11-26	T5192	WB	IF	IHC	IP
	sigma	x1000
	T5192
	rabbit

	Drosophila embryo, discs, nervous system
α-Tubulin2011-09-27	B-5-1-2	WB	IF	IHC	IP
	SIGMA	1:2000~1:5000	1:1000
	T6074
	mouse
	m	IF: 固定、permeabilizeは37℃で行う
	MEF, HeLa, A549, MCF7他
α-Tubulin2011-10-17	DM1A	WB	IF	IHC	IP
	Sigma	X1000	X500
	T6199
	mouse	【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	Mono	WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	HeLa細胞
g-tubulin2011-08-29	GTU-88	WB	IF	IHC	IP
	SIGMA		x1000
	T6557
	mouse	【PFA+Triton】:○,【IS】:○,【TCA】:◎【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	mono, IgG1	セミカルバジドでquencing,またはCanGetSignalA (from TOYOBO Inc.)でみえる Quenching by Semicarbazide is needed. Or use CanGetSignalA (from TOYOBO Inc.)
	Hela細胞
γ-Tubulin2011-10-03	GTU-88	WB	IF	IHC	IP
	sigma	x1000	x500
	T6557
	mouse	【Formalin in PBS+0.05%Triton】:○
	Mono, IgG1	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
γ-Tubulin2011-10-13	GTU-88	WB	IF	IHC	IP
	SIGMA		X500
	T6557
	mouse
	Mono, IgG1
	HeLa細胞
tubulin(γ)2011-11-26	GTU88	WB	IF	IHC	IP
	Sigma	x3000
	T6557
	mouse

	Drosophila embryo, discs, nervous system
Tubulin, Acetylated2011-09-27		WB	IF	IHC	IP
	Sigma		100
	T6793
	mouse
	m	4%PFA>MeOH, TCA
	MDCK II
Acetylated Tubulin2011-08-10		WB	IF	IHC	IP
	SIGMA		x100
	T7451	3%PFA (in PBS)
	mouse
	m
	マウス組織細胞など
Acetylated tubulin2011-10-03	6-11B-1	WB	IF	IHC	IP
	sigma		×50000
	T7451
	mouse	【Formalin in PBS+0.05%Triton】:○
	Mono	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
α-Tubulin2011-08-10	clone:DM1A	WB	IF	IHC	IP
	Sigma		x100
	T9026	3%PFA (in PBS)
	mouse
	m
	マウス脳など
α-Tubulin2011-09-29	DM1A	WB	IF	IHC	IP
	SIGMA	x10,000~	x50~100
	T9026
	mouse	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG1
	HeLa細胞
α-Tubulin2011-09-27	clone DM1A	WB	IF	IHC	IP
	Sigma		200-500
	T9026
	mouse
	m	4%PFA, MeOH, TCA
	試した限り全ての培養細胞、組織
tubulin2011-11-26	DM1A	WB	IF	IHC	IP
	Sigma	x1000
	T9026
	mouse

	Drosophila embryo, discs, nervous system
Tubulin, Tyrosine2011-10-03	TUB-1A2	WB	IF	IHC	IP
	Sigma		×1500
	T9028
	mouse	【Formalin in PBS+0.05%Triton】:○
	Mono,IgG3	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons

【U】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
utrophin2011-10-14	―	WB	IF	IHC	IP
	Santa Cruz	x 1,000	x 1,000
	UT-2	2％ PFA 0.5%Triton, RT 17min
	rabbit
	Poly	【抗原の種、部位】human 1768-2078 a.a.
	MDCK cells

【V】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Vimentin2011-08-29		WB	IF	IHC	IP
	SySy		ダメ

	rabbit	【PFA+Triton】:×【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Poly
	Hela細胞

【v】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Vimentin (Chicken)2016-08-10	AMF-17b	WB	IF	IHC	IP
	Developmental Studies Hybridoma Bank (DSHB)		x300
		4%PFA (in phosphate buffer)
	Mouse	0.1% TritonX-100/PBS
	IgG1
	MDCK cell

【V】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Vinculin2011-10-13 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam	X500	X200
	ab11194
	mouse	PFA+Triton or MeOH
	IgG1
	HeLa細胞、TIG-1細胞
human Vimentin2011-08-10		WB	IF	IHC	IP
	Chemicon
	CBL202
	mouse
	m

human Villin2011-08-10	C-19	WB	IF	IHC	IP
	Santa Cruz		x100
	sc7672	3%PFA (in PBS)
	goat
	p	染色薄い Weak staining
	マウス小腸
Vinculin2011-09-27	clone VIN 11-5	WB	IF	IHC	IP
	Sigma		100
	V4505
	mouse
	m	1%PFA, MeOH > TCA > 4%PFA
	試した限り全ての培養細胞
vinculin2011-10-14	hVIN-1	WB	IF	IHC	IP
	Sigma		x 500
	V9131	2％ PFA 0.5%Triton, RT 15min, methanol 固定可能 Methanol fixation also applicable
	mouse
	Mono、IgG1	【抗原の種、部位】human whole protein
	MDCK cells

【Z】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Zip Kinase2011-09-27		WB	IF	IHC	IP
	Sigma		100
	Z0134
	rabbit
	p	1%PFA on ice
	MDCK II