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Antibody【Antigen:P-T】|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody【Antigen:P-T】

【P】

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
aPKC (zeta and lambda)2011-10-14 WBIF IHCIP
Santa Cruz x 1000x 300
sc-216(C-20)
2% PFA 0.5%Triton, RT 15min
rabbit
Poly
【抗原の種、部位】rat C-terminus
MDCK cells
aPKC iota2011-10-14 23/PKCiWBIF IHCIP
BD Bioscience x 1000x 100
610176
2% PFA 0.5%Triton, RT 15min
mouse
Mono、IgG2b
【抗原の種、部位】human a.a. 404-587
aPKC iota はllamdaと同じである
APKC iota is the same as aPKC lamda
MDCK cells
human p75 pAb2011-08-10 WBIF IHCIP
Promegax100
G3231
3%PFA (in PBS)
rabbit
神経らしき線維が染まる
Axon-like fibers are detected by this Ab.
マウス小腸
human p75 pAb2011-08-12 WBIF IHCIP
Promega
G3231
rabbit
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human p75 pAb2012-08-03 WBIF IHCIP
Promegax100
G3231
3%PFA in PBS
rabbit
0.05% saponin

PC12
human PCNA2011-08-10 FL-261WBIF IHCIP
Santa Cruz
sc7907
rabbit
p

human PepT12011-08-10 H-235WBIF IHCIP
Santa Cruzx100
sc20653
methanol
rabbit
p
メタノール固定で染まる、WBはOK
Methanol fixation is OK for IF. Also applicable for WB
マウス小腸
human PepT12011-08-10 WBIF IHCIP
Acrisx100
R1532P
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
human Pin12011-08-10 H-123WBIF IHCIP
Santa Cruz
sc15340
rabbit
p

未使用
Not used so far
human PKA b cat2011-08-10 C-20WBIF IHCIP
Santa Cruz
sc904
rabbit

human PKC m2011-08-10 D-20WBIF IHCIP
Santa Cruz
sc935
rabbit
p

human PKC mu2011-08-10
About Product
PolyclonalWBIF IHCIP
Abcam
ab5284
rabbit
IgG

human PKD22011-08-10 BL844WBIF IHCIP
Bethyl
A300074A
rabbit

human podocalyxin2011-08-10 WBIF IHCIP
R&D Systemsx100
AF1658
methanol
goat
メタノール固定で弱く染まる?
Weak staining by methanol fix.
MDCK
human PP1 alpha2011-08-10 WBIF IHCIP
Biocomparex1000
2581S
rabbit
p

マウス脳
human PP1 delta2011-08-10 WBIF IHCIP
Upstatex1000
7270
rabbit

マウス脳
mouseαPCNA2011-08-10 WBIF IHCIP
DakoCytomationx100
M0879
3%PFA (in PBS)
mouse
m

マウス胎児脳
NGF-receptor (p75)2012-08-03 WBIF IHCIP
NeoMarkersx100
3%PFA in PBS
mouse
0.05% saponin

PC12
p1152012-05-21 WBIF IHCIP
BD Transduction Labx100
P67420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
p150 Glued2011-08-14 WBIF IHCIP
BD transduction
P41920
mouse
m

p150Glued2011-09-29 12WBIF IHCIP
BD Transduction Lab.x200
612709
mouse
IF: -20℃ methanol or acetone:methanol=1:1
mono, IgG1

HeLa細胞
p150glued2011-10-06 12/P150GLUEDWBIF IHCIP
BD biosciencex400
612709
mouse
4%PFA+0.1%Saponin :○
IgG1

HeLa
p17(HIV-1 a-MA)2011-08-29 13-103-1-100WBIF IHCIP
ABI
mouse
Mono, IgG1

Hela細胞
p2302011-08-29 WBIF IHCIP
BD transductionx250x200
mouse
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1
MDCKII OK
Hela細胞
p2302011-09-27 WBIF IHCIP
BD Biosciences1:100
611280
mouse
m
trans Golgi
HeLa, A549, MCF7他
p230tranGolgi2012-05-21 WBIF IHCIP
BD Transduction Labx100
G88620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
p24(HIV-1 a-CA)2011-08-29 13-102-100WBIF IHCIP
ABI
mouse
Mono, IgG1

Hela細胞
p272012-12-12 WBIF IHCIP
BD pharmingenx100
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
G1
mouse brain
p47A2011-08-29 clone 18WBIF IHCIP
BD Transductionx250
mouse
Mono, IgG1

Hela細胞
p47A2013-07-04 WBIF IHCIP
BD transductionx100
610900
3%PFA (in PBS)
mouse
0.05%saponin
IgG1
fine punctate staining like adaptin alpha , included in Coated vesicle sampler kit
Caco2細胞

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
p47A2014-04-03 WBIF IHCIP
BD Transductionx100
610900
3%PFA (in phosphate buffer)
mouse
IgG1

mouse small intestine

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
p532011-10-13 DO-1WBIF IHCIP
ThermoX 100
MS-187-PO
mouse
Mono, IgG2a

TIG-1細胞
p622011-08-14 WBIF IHCIP
MBL
PM045
rabbit

p622011-08-29 WBIF IHCIP
Progenx1000x100
guinea pig
Poly

Hela細胞
p62/SQSTM2011-09-27 WBIF IHCIP
MBL1:20001:500
PM045
rabbit
p

MEF, HeLa, A549, MCF7他

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
p632017-10-10 WBIF IHCIP
abcam1/500
ab97865
4% PFA at 4oC over night
Rabbit
0.5% Triton in PBS

zebrafish larvae (1 dpf/ day post fertilization)

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
p70S6 Kinase2011-09-27 WBIF IHCIP
Cell Signaling1:1000
#9202
rabbit
p

PACS-1a2011-08-29 clone 4WBIF IHCIP
BD Transductionx200ダメ?
mouse
【PFA+Saponin】:×【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
PACS12011-08-29 60B233WBIF IHCIP
Antageneダメ?
rabbit
【PFA+Saponin】:×【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS
poly

Hela細胞
PAK12011-10-04 WBIF IHCIP
Santa CruzX1000
SC882
rabbit
Poly

HL-60細胞
PAK2(C17A10)2011-10-17 WBIF IHCIP
Cell SignalingX1000
#2615
rabbit
Mono, IgG
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
PAR-1a/MARK3/c-TAK1 2011-10-14 183.A10.A3WBIF IHCIP
Millipore/Upstate x 300
05-680
2% PFA 0.5%Triton, RT 15min
mouse
Mono, unknown
【抗原の種、部位】human full length
MDCK cells
PAR-1b/MARK2/EMK 2011-10-14 WBIF IHCIP

PAR-32011-09-27 WBIF IHCIP
Millipore (Upstate)100
07-330
rabbit
p
1%PFA, TCA
DLD-1, EpH4, MTD-1A
PAR-32011-10-14 WBIF IHCIP
Millipore/Upstate x 500x 500
07-330
2% PFA 0.5%Triton, RT 15min
rabbit
Poly
【抗原の種、部位】mouse 457-744 a.a
MDCK cells
PAR-4/LKB2011-10-14 WBIF IHCIP

PAR-5/14-3-3 (pan)2011-10-14 WBIF IHCIP
Santa Cruz x 500
sc-629 (K-19)
2% PFA 0.5%Triton, RT 15min
rabbit
Poly
【抗原の種、部位】human N-terninal
MDCK cells
PAR-5/14-3-3 b/z2011-10-14 WBIF IHCIP
Stressgenx 1000
KAM-CC012
mouse
Mono, unknown
【抗原の種、部位】lysed trransformed cells
PAR-6β2011-10-14 WBIF IHCIP
2% PFA 0.5%Triton, RT 15min

MDCK cells
PAR32011-08-14 WBIF IHCIP
upstatex100
07-330
3%PFA (in PBS)
rabbit
p

マウス小腸
Par32011-10-03 WBIF IHCIP
upstatex500
07-330
rabbit
【Formalin in PBS+0.05%Triton】:○
poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Parvalbumin2012-12-12 WBIF IHCIP
Chemiconx100
mab1572
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
a type of interneuron, centrosome?
mouse brain
Parvalbumin2012-12-12 WBIF IHCIP
Sigmax100
P3088
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
a type of interneuron, centrosome?
mouse brain
patched (ptc)2011-11-26 Apa1WBIF IHCIP
Developmental study hybridoma bank C
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Pax62012-12-12 WBIF IHCIP
Chemiconx200
AB5409
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
dorsal neural progenitors
mouse brain
PCNA2011-10-13 5A10WBIF IHCIP
MBLX10000
MH-12-3
mouse
Mono, IgG1

HeLa細胞
PCNA2012-12-12 WBIF IHCIP
Oncogene researchx200
NA03
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min

mouse brain
PDGFreceptor alpha chain2012-12-12 WBIF IHCIP
BD pharmingenx500
558774
4%PFA (in PBS)
rat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG2ak
Oligodendrocyte
mouse brain
PDI2011-08-29 1D3WBIF IHCIP
StressGen
mouse
【PFA+Triton】:○,【PFA+Saponin】:×,【MeOH】:○,【TCA】:△【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
PDI2012-01-25 WBIF IHCIP
BD Transductionx100
P71720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
PDI2012-03-02 WBIF IHCIP
Stressgenx100
SPA-891
methanol
rabbit
3%PFA in PBSでも染まるが弱い
Only weakly stained by 3%PFA in PBS fixation
mouse embryonic fibroblast (MEF)
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
PDI2012-11-13 WBIF IHCIP
BD Transductionx100
P71720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

Caco2細胞
PDI2012-11-13 WBIF IHCIP
BD Transductionx100
P71720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
PDI2013-01-19 1D3WBIF IHCIP
Stressgenx100
SPA891
3%PFA (in PBS)
mouse
0.05%saponin
IgG1
そまる細胞の割合は他のER markerより高い
Higher rate of cells are stained by this Ab than other ER Abs.
Caco2細胞

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PDI2014-02-04 WBIF IHCIP
StressGenx100
SPA891
3%PFA (in PBS)
mouse
0.05%saponin

MDCK

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Peanut2011-11-26 4C9H4WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse
IgG

Drosophila embryo, discs, nervous system
Pep122011-08-31 IgG1WBIF IHCIP
Invitrogen (Molecular Probes)
A-21273
Mouse
monoclonal

Pericentrin2011-09-27 WBIF IHCIP
Covance100
PRB-432C
rabbit
p
MeOH, TCA
MDCK II
Pericentrin2018-07-26 WBIF IHCIP
abcam1/2000
ab4448
MeOH, PFA
Rabbit
0.05% triton X-100 for PFA fixation

RPE1, HeLa, U2OS
pericentrin2018-07-26 WBIF IHCIP
abcam1/1000
ab28144
MeOH fixation
Mouse
0.05% triton X-100

HeLa
pericentrin2018-07-26 WBIF IHCIP
abcam1/1000
ab4448
MeOH fixation
Rabbit
0.05% triton X-100

HeLa
PERK2011-09-17 C33E10WBIF IHCIP
Cell Signaling
#3192
rabbit
Mono

Hela細胞
PERK2011-09-28 C33E10WBIF IHCIP
Cell Signalingx250
#3192
rabbit
Mono

HeLa細胞
PERK2019-09-10 C33E10WBIF IHCIP
Cell Signaling (CST)x1000
#3192
Rabbit
IgG

HepG2
pH32012-12-12 WBIF IHCIP
Cell Signalingx400
9706
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
mitosis
mouse brain
phospho p42/442011-09-22 WBIF IHCIP
Cell Signaling Tech1/50-1/100
9101
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly
incubate w/ Phosphatase inhibitors
mouse E6.5 embryo.MEF
phospho p70S6 Kinase (T389)2011-09-27 WBIF IHCIP
Cell Signaling1:1000
#9205
rabbit
p

Phospho-eIF2alpha(Ser51)2011-09-17 D9G8WBIF IHCIP
Cell Signalingx1000
#3398
rabbit
Mono

Hela細胞
Phospho-eIF2alpha(Ser51)2011-09-28 D9G8WBIF IHCIP
Cell Signalingx1000
#3398
rabbit
Mono

HeLa細胞

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-ERK(Thr202/Tyr204)2014-10-06 197G2WBIF IHCIP
Cell Signalingx100
4377
4%PFA (in phosphate buffer)
rabbit
Use 'Can Get Signal'(TOYOBO) for staining
Drosohila imaginal disc
phospho-Histone H3 (Ser10)2017-10-10 WBIF IHCIP
Cell Signaing1/100
9701
4% PFA at 4oC over night
Rabbit
0.5% Triton in PBS

zebrafish larvae (1 dpf/ day post fertilization)
phospho-Histone H3(S10)2014-09-04 WBIF IHCIP
Cell Signalingx100
9701
4%PFA (in phosphate buffer)
rabbit

Drosohila imaginal disc
phospho-JNK(Thr183/Tyr185) 2014-09-04 G9WBIF IHCIP
Cell Signalingx100
9255
4%PFA (in phosphate buffer)
mouse
IgG1
Use 'Can Get Signal'(TOYOBO) for staining
Drosohila imaginal disc

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Phospho-PERK (Thr980)(16F8)2019-09-10 16F8WBIF IHCIP
Cell Signaling (CST)x1000
#3179
Rabbit
IgG

マウス大腸
Phospho-PERK(Thr980)2011-09-17 16F8WBIF IHCIP
Cell Signalingx1000
#3179
rabbit
Mono

Hela細胞
Phospho-PERK(Thr980)2011-09-28 16F8WBIF IHCIP
Cell Signalingx1000
#3179
rabbit
Mono

HeLa細胞
phospho-PKA substrate2011-08-14 WBIF IHCIP
Cell Signaling
9621S
rabbit
p

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Smad1/5/82017-09-14 WBIF IHCIP
Cell Signaling1/200
9511
4% PFA at 4oC over night
Rabbit
MeOH at -20oC over night

zebrafish embryos (6 hpf/hours post fertilization)
phospho-Smad22017-09-14 WBIF IHCIP
Invitrogen1/200
44-244G
4% PFA at 4oC over night
Rabbit
MeOH at -20oC over night

zebrafish embryos (8-10 hpf/hours post fertilization)
phospho-Src(Y418)2014-10-06 WBIF IHCIP
Invitrogenx100
44660G
4%PFA (in phosphate buffer)
rabbit

Drosohila imaginal disc

【p】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Phosphoglycerate Kinase2011-08-31 IgG1WBIF IHCIP
Invitrogen (MitoSciences)
A-6457
Mouse
monoclonal

Phosphorylated-Tyrosine(p-Tyr)2011-11-26 PY-99WBIF IHCIP
Santa Cruzx50
sc7020
4%PFA +0.1%Triton
mouse IgG2b
IgG2b

Drosophila embryo, discs, nervous system
PhosphoTyrosine2011-08-29 4G10WBIF IHCIP
Upstatex1000
mouse
Mono

Hela細胞
PI 4-kinase?α2011-08-29 WBIF IHCIP
ABGENTx100-ダメ
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
PI 4-kinase?α2011-08-29 WBIF IHCIP
ABGENTダメ?ダメ
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
PI 4-kinase?β2011-08-29 WBIF IHCIP
ABGENTx100-ダメ
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
PI 4-kinase?β2011-08-29 clone 7WBIF IHCIP
BD Pharmingenx500x5000-10000
mouse
【PFA+Triton】:◎,【PFA+Saponin】:◎,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a

Hela細胞
PI 4-kinase?β2011-08-29 WBIF IHCIP
Upstatex500-x400-
rabbit
【PFA+Triton】:○,【PFA+Saponin】:◎,【IS】:△,【MeOH】:△【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
Saponinがbetter。
Can get better staining by saponin permeabilization.
Hela細胞
PI(4)P2011-08-29 WBIF IHCIP
Echelonx500-
mouse
【PFA+Triton】:×,【PFA+Saponin】:○,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgM

Hela細胞
PI(4,5)P22011-08-29 WBIF IHCIP
Echelonx100
mouse
Mono, IgM
PFA+Saponin(+biotin+streptavidin)でも微妙
Very weak staining by PFA+Saponin(+biotin+streptavidin)
Hela細胞
PI3 kinase p852011-10-03 WBIF IHCIP
upstatex1000
06-497
rabbit
poly

rat hippocampal neurons
PI3 kinase p852011-10-03 4WBIF IHCIP
BDx500
610045
mouse
Mono, IgG2a

rat hippocampal neurons
PIK3R4 (p150)2011-09-27 1G12WBIF IHCIP
Abnova1:1000
H00030849-M03
mouse
m

PIP22011-10-06 2C11WBIF IHCIP
LifeSpanBIosciencex400
LS-C49060-100
mouse
1% Glutalaldehyde + 4%PFA + 0.1%Saponin
IgM
Glutaladehyde固定は必須。固定後すぐに次のステップへ。
HeLa
PIP32011-10-06 RC6F8WBIF IHCIP
Invitrogenx400
A-21328
mouse
1% Glutalaldehyde + 4%PFA + 0.1%Saponin
IgM
Glutaladehyde固定は必須。固定後すぐに次のステップへ。
HeLa
PKA RIIβ2011-08-14 WBIF IHCIP
BD transductionx1000x100
P54720
3%PFA (in PBS)
mouse
m

マウス神経細胞
PKA RIα2011-08-14 WBIF IHCIP
BD transduction
P53620
mouse
m

PKA RIα2011-08-14 WBIF IHCIP
BD transduction
P19920
mouse
m

PKARIIalpha2011-08-29 40WBIF IHCIP
BD Transduction Laboratoriesx10000
mouse
Mono, IgG1

Hela細胞
PKARIIbeta2011-08-29 45WBIF IHCIP
BD Transduction Laboratoriesx10000
mouse
Mono, IgG1

Hela細胞
PKC delta2011-09-17
About Product
MonoclonalWBIF IHCIP
Abcamx2000
ab108972
rabbit
IgG1

Hela細胞
PKC delta2011-09-28
About Product
MonoclonalWBIF IHCIP
Abcamx2000
ab108972
rabbit
IgG1

HeLa細胞

【P】

Antigen
last update
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Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKC zeta (C-20)2014-09-04 WBIF IHCIP
Santa Cruzx500
sc-216
4%PFA (in phosphate buffer)
rabbit

Drosohila imaginal disc

【p】

Antigen
last update
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Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKCm2011-08-29 C-20(sc-639)WBIF IHCIP
Santa Cruzx500
rabbit
Poly

Hela細胞
PKCzeta2011-09-27 WBIF IHCIP
Santa Cruz100
sc-216
rabbit
p
1%PFA, TCA
DLD-1, EpH4, MTD-1A

【P】

Antigen
last update
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Isotype
Comments
Samples
Reference
PKD 12014-01-07 WBIF IHCIP
Cell Signaling Technologyx500x100
2052
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung
PKD 12014-01-07 WBIF IHCIP
Sigmax100000x100
P 3987
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung
PKD 12014-01-07 WBIF IHCIP
St. Cruz Biotechnology C-20x100000x100
SC-639
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung
PKD 12014-01-07 WBIF IHCIP
St. Cruz Biotechnology D-20x2000x100
SC-935
3%PFA (in PBS)
rabbit
IgG
WB: cross reaction with PKD2
WB: several tissues; IF: Lung
PKD 32014-01-07 WBIF IHCIP
Cell Signaling Technologyx1000
5655
rabbit
IgG

WB: several tissues

【p】

Antigen
last update
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Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PKD22011-08-14
About Product
MonoclonalWBIF IHCIP
Abcam
ab51250
rabbit
IgG

PLD2011-08-29 WBIF IHCIP
Upstate
rabbit
Poly

Hela細胞
Plk12011-10-17 35-206WBIF IHCIP
invitrogenX500
37-7000
mouse
Mono, IgG1-kappa
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
Plk12018-07-26 WBIF IHCIP
Bethyl laboratories1/1000
A300?251A
MeOH fixation
Rabbit
0.05% triton X-100

HeLa
Plk42011-10-17 WBIF IHCIP
Cell SignilingX1000X100
#3258
rabbit
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
Plk42018-07-26 6H5WBIF IHCIP
Merck Millipore1/500
MABC544
MeOH fixation
Mouse
0.05% triton X-100

HeLa

【P】

Antigen
last update
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MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
podocalyxin2014-02-04 WBIF IHCIP
R&D systemsx100
AF1658
3%PFA (in PBS)
goat
0.05%saponin

MDCK

【p】

Antigen
last update
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MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Polyglutamylated tubulin2018-08-09 GT335WBIF IHCIP
AdipoGen1/500
AG-20B-0020-C100
MeOH
Mouse

RPE1, HeLa, U2OS
Polyglutamylation Modification2018-07-26 GT335WBIF IHCIP
AdipoGen1/1000
AG-20B-0020-C100
MeOH fixation
Mouse
0.05% triton X-100

HeLa
Porin2011-08-31 IgG1WBIF IHCIP
Invitrogen (MitoSciences)
459500
Mouse
monoclonal

PP12011-08-10 protein phosphatase 1WBIF IHCIP
BD Transductionx1000
610373
mouse
m

マウス脳
PP2A2011-08-14 WBIF IHCIP
BD transductionx1000
610555
mouse
m

マウス脳
pPAK(Thr423)2011-10-04 WBIF IHCIP
InvitrogenX1000
44-942G
rabbit
Poly

HL-60細胞
pro-SPC2012-02-21 WBIF IHCIP
Seven Hills Bioreagentsx100
WRAB-9337
3%PFA in 0.1M phosphate buffer (pH7.2)
rabbit
還流固定
なじみのない会社なので注文には時間がかかるかもしれません
Ordering may take time because this Ab is produced by a small company.
mouse lung frozen section
prospero2011-11-26 MR1AWBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila nervous system
Protein Kinase D2011-08-10 WBIF IHCIP
SIGMA
P3987
rabbit

Prox12012-12-12 WBIF IHCIP
abcamx2000x1000
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain, primary hippocampal neuron
Prox12012-12-12 WBIF IHCIP
RELIA Tech GmbHx1000
102-PA32AG
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain
Prox12012-12-12 WBIF IHCIP
Upstatex1000
07-537
rabbit
RIPA
IgG
DG, svz in GE, interneuron
mouse brain lysate
Prox12012-12-12 4G10WBIF IHCIP
Chemiconx1000
MAB5654
mouse
RIPA
IgG2bk
DG, svz in GE, interneuron
mouse brain lysate
Prox12012-12-12 WBIF IHCIP
Covancex500
PRB-238C
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain
Prox12012-12-12 WBIF IHCIP
R and D systemsx2000x1000
AF2727
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain, primary hippocampal neuron
Prox12012-12-12 WBIF IHCIP
Chemiconx500
AB5475
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain
PSA-NCAM2012-12-12 WBIF IHCIP
Chemiconx200
MAB5324
ice Methaol
mouse
IgM
immature neuron
mouse brain

【P】

Antigen
last update
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MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
PTP10D (drosophila)2016-08-10 8B22F5WBIF IHCIP
Developmental Studies Hybridoma Bank (DSHB)x100
4%PFA (in phosphate buffer)
Mouse
0.1% TritonX-100/PBS
IgG1

Drosohila imaginal disc
Antigen
last update
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MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Rabbit polyPericentrin co.VANCE2011-08-10 WBIF IHCIP
COVANCEx100
prb432c
3%PFA (in PBS)
rabbit
p

マウス胎児脳
rabbitαPax62011-08-10 WBIF IHCIP
COVANCEx100
prb278p
3%PFA (in PBS)
rabbit
p

マウス胎児脳
rabbitαPKCμ2011-08-10 C-20WBIF IHCIP
Santa Cruz
sc639
rabbit
p

rat PDI2011-08-10 WBIF IHCIP
Stressgen
SPA891
3%PFA (in PBS)
mouse
m

IFOK?
マウスMEF
rat PKC g2011-08-10 WBIF IHCIP
Zymedx100
133800
3%PFA (in PBS)
mouse

マウス小脳
rat prolactin2011-08-10 PRLWBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACRT2601RBP85
3%PFA (in PBS)
rabbit

マウス下垂体

【R】

【r】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
c-Raf-12013-09-12 53/c-Raf-1WBIF IHCIP
BD Transduction Laboratoriesx1000
610151
mouse
IgG1

HeLa
human Rab112011-08-10 C-19WBIF IHCIP
Santa Cruzx100
sc6565
3%PFA (in PBS), methanol
goat
p

マウス小腸
human Rab62011-08-12 C-19WBIF IHCIP
Santa Cruz
sc310
rabbit
p
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human Rab62011-08-10 C-19WBIF IHCIP
Santa Cruzx100
sc310
3%PFA (in PBS)
rabbit
p

マウスMEF、WB
human Rab6 (C-19)2012-08-03 WBIF IHCIP
Santa Cruzx100
sc310
3%PFA in PBS
rabbit
0.05% saponin

PC12
human Rab72011-08-10 H-50WBIF IHCIP
Santa Cruzx100
sc10767
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
human Rab9A2011-08-10 C-18WBIF IHCIP
Santa Cruzx100
sc6562
3%PFA (in PBS), methanol
goat
p

マウス小腸
N-Ras2011-10-13 F115-227WBIF IHCIP
CALBIOCHEMX 500
OP25
mouse
Mono, IgG1

HeLa細胞
pan-Ras2013-09-12 Ab-3WBIF IHCIP
Oncogenex100
OP#40
mouse
IgG2a

HeLa
Rab 42011-08-29 D-20WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
Rab 5A2011-08-29 sc-309WBIF IHCIP
Santa Cruzx100
rabbit
Poly

Hela細胞
Rab 5B2011-08-29 A-20WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
Rab112011-08-14 WBIF IHCIP
BD transduction
610656
mouse
m

Rab112011-08-14 WBIF IHCIP
Zymed
71-5300
rabbit
p

Rab112011-08-29 WBIF IHCIP
Zymedx500x100-
rabbit
【PFA+Triton】:△,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
WB:Rab11aのみ認識、IFで見えるendosomeのシグナルはKDで半分ぐらいしか消えない。
Only recognize Rab11a by WB. Only half of the endosome staining dissapears in Rab11a KD cells.
Hela細胞
Rab112011-08-29 clone 47WBIF IHCIP
BD Transductionx200-x200
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×,【TCA】:○、◎(+CanGetSignal (from TOYOBO Inc.))、BlockingはO/N【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a
WB: Rab11a, b両方認識する
Recognize both Rab11a and Rab11b by WB.
Hela細胞
Rab112011-08-31 WBIF IHCIP
Invitrogen
71-5300
4%PFA (in PBS)
rabbit
p
Rab11A,B共に認識
Recognize both Rab11a and Rab11b
マウス海馬ニューロン, MEF
Rab112011-08-31 WBIF IHCIP
BD transduction
610656
4%PFA (in PBS)
mouse
m
Rab11A,B共に認識
recognize both Rab11a and Rab11b
MEF
Rab112011-09-27 clone 47/Rab11WBIF IHCIP
BD100
610656
mouse
m
TCA (Can Get Signal Immunostain 使用)
http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld96/technical/canget96tr01.pdf
http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld95/technical/canget95tr02.pdf
HeLa
Rab142011-08-29
About Product
PolyclonalWBIF IHCIP
Abcamx500x100
ab40938
rabbit
IgG
Saponinがbetter 汚い
Saponin permeabilization is better.
Hela細胞
Rab1A2011-08-31 WBIF IHCIP
Abnova
H00005861-M07A
mouse
m

Rab272011-08-14 WBIF IHCIP
BD transduction
610596
mouse
m

Rab272011-08-31 WBIF IHCIP
IBL
18975
4%PFA (in PBS)
rabbit
p
Rab27A,B共に認識
recognize both Rab27a and Rab27b
melan-a
Rab27A2011-08-31 WBIF IHCIP
Abnova
H00005873-M02
mouse
m

Rab32011-08-31 WBIF IHCIP
BD transduction
610379
4%PFA (in PBS)
mouse
m

マウス海馬ニューロン, PC12
rab33a2011-10-03 8A11WBIF IHCIP
Abnovax1000
H00009396-M04
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer+0.05%Triton】:○
Mono,IgG2ak

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Rab33B2011-08-31 WBIF IHCIP
Frontier Science
D5
4%PFA (in PBS)
mouse
m
MEF, NIH3T3ではIF不可。Rab33Aは認識せず。
Not suitable for IF in MEFs and 3T3. Not recognize Rab33A.
HeLa, HeLaS3
Rab352011-08-31 WBIF IHCIP
ProteinTech Group
11329-2-AP
rabbit
p

Rab42011-08-14 WBIF IHCIP
Stressgen
KAP-GP005
rabbit
p

Rab42011-08-29 clone 7WBIF IHCIP
BD Transductionx100-ダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1
endosome likeのsignalが見えるが、siRab4で消えない。
Recognize endosome-like signals which cannot disappear by siRab4.
Hela細胞
rab42011-09-22 WBIF IHCIP
Stressgen1/500
KAP-GP005
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
serum

mouse E6.5 embryo.MEF
Rab52011-08-14 WBIF IHCIP
BD transduction
R28720
mouse
m

Rab52011-08-29 clone 1WBIF IHCIP
BD Transductionx200-x100-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a
PFAでもいいが、saponinがbetter
Saponin permeabilization is better.
Hela細胞

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
rab52014-10-06 WBIF IHCIP
Abcamx500
ab31261
4%PFA (in phosphate buffer)
rabbit

Drosohila imaginal disc
rab52016-08-10 621.3WBIF IHCIP
Synaptic systems1/10001/500
108011
4% PFA
Mouse
IgG

HeLa, PC12

【r】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Rab5 (S-19)2011-10-17 WBIF IHCIP
Santa CruzX500
sc-309
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
Rab6A2012-04-17 38-TBWBIF IHCIP
SantaCruzx1,000
sc-81913
mouse

Rab72011-08-14 WBIF IHCIP
MBL
rabbit
p

Rab72011-08-29 Rab7-117WBIF IHCIP
Sigmaダメ
R8779
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2b

Hela細胞
Rab72011-08-29 WBIF IHCIP
Sigmaダメ
R4779
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
Rab72011-08-31 WBIF IHCIP
Cell signaling
2094S
4%PFA (in PBS)
rabbit
p

melan-a
Rab72016-12-14 WBIF IHCIP
Cell signaling1/10001/500
9367
4% PFA
Rabbit
IgG

HeLa, PC12
Rab82011-08-14 WBIF IHCIP
BD transductionx1000x100
610845
3%PFA (in PBS), methanol
mouse
m
Rab8a,b共に認識
Recognize both Rab8a and Rab8b
マウス小腸
Rab82011-08-29 WBIF IHCIP
Sigmax300-500
R5530
rabbit
【PFA+Triton】:○,【TCA】:◎【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
Poly, anti-sera,IgGfraction

Hela細胞
Rab82012-04-17 4/Rab4WBIF IHCIP
Transductionx5,000~
61-844
mouse
IgG2b

Rab82012-04-17 WBIF IHCIP
SantaCrusx500-1,000
sc-26578
goat

Rab82012-05-21 WBIF IHCIP
BD Transduction Labx100
R66320
3%PFA in PBS
mouse
0.05% saponin
IgG2b
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
Rab82012-05-21 WBIF IHCIP
BD Transduction Labx100
R66320
3%PFA in PBS
mouse
0.05% saponin
IgG2b
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
Rabaptin-52011-08-29 clone 20WBIF IHCIP
BD Transductionダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
Rabaptin52011-08-29 C-20WBIF IHCIP
Santa Cruzx500-1000x50
goat
Poly

Hela細胞
Rabaptin52011-08-29 N-19WBIF IHCIP
Santa Cruz
goat
Poly

Hela細胞
Rabenosyn-52011-08-29
About Product
PolyclonalWBIF IHCIP
Abcamダメ?ダメ
ab21196
goat
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
IgG

Hela細胞
Rac12011-08-29 WBIF IHCIP
Chemicon
mouse
Mono

Hela細胞
Rac12011-08-29 clone 102WBIF IHCIP
BD Pharmingenダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2b

Hela細胞
Rac12011-09-27 clone 23A8WBIF IHCIP
Upstate100
05-389
mouse
m
4%PFA, TCA
HeLa, MDCK II, DLD-1, EpH4, MTD-1A
Rac12011-10-04 23A8WBIF IHCIP
MilliporeX2000
05-389
mouse
Mono, IgG2b

HL-60細胞
Rac22011-10-04 WBIF IHCIP
UpstateX5000
07-604
rabbit
Poly

HL-60細胞
RACGAP12011-09-27 clone 1G6WBIF IHCIP
Abnova100
H00029127-M01
m
m
MeOHでmidobodyのみわかる程度で弱い
Only midbody can be weakly visualized by MeOH fix.
HeLa
RACGAP1/MgcRacGAP2011-09-27 WBIF IHCIP
IMGENEX100
IMG-3464
goat
p
弱い(midbodyのみ)
Only midbody can be weakly visualized.
HeLa
RagC2017-08-02 D31G9WBIF IHCIP
Cell Signaling Technology1/1000
5466
Rabbit

HEK293, HeLa
Ran2011-10-13 20WBIF IHCIP
BDX2000~
610340
mouse
PFA+Triton or MeOH or TCA
Mono, IgG2a

HeLa細胞
RanGAP-12012-01-19 19C7,monoclonalWBIF IHCIP
Invitrogen X300-X500
33-0800
PFA, Triton
mouse
IgG1

HeLa細胞
Ras2014-10-06 WBIF IHCIP
Cell Signalingx100
3965
4%PFA (in phosphate buffer)
rabbit

Drosohila imaginal disc
rat ROMK12011-08-10 Kir1.1, Kcnj1WBIF IHCIP
Alomonex100
APC001
3%PFA (in PBS)
rabbit

マウス腎臓
rat ROMK12011-08-10 Kir1.1, Kcnj1WBIF IHCIP
Alomonex100
APC001
3%PFA (in PBS)
rabbit

マウス腎臓
Rcc12011-10-13 N-19WBIF IHCIP
Santa Cruz
sc-1161
goat
Poly

HeLa細胞
Repo2011-11-26 8D12WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila nervous system
Rer12014-06-19 WBIF IHCIP
Sigma-Aldrichx1000x200
R4407
3%PFA (in PBS)
Rabbit
0.05% Saponin
IgG1
highly specific for human by WB and IF
HeLa
RFP2011-08-29 8D6WBIF IHCIP
MBL
mouse
Mono, IgG1
良くない、WBにはPolyclonalを使うこと
Not good. Use polyclonal Ab for WB
Hela細胞
RFP2011-08-29 3G5WBIF IHCIP
MBL
mouse
Mono, IgG1
IP用
For IP only.
Hela細胞
RFP2011-08-29 WBIF IHCIP
MBLx1000x1000
rabbit
Poly
mCherryに使える
Also applicable for staining mCherry.
Hela細胞
Rho2011-09-27 clone EP487YWBIF IHCIP
EPT100
1667-1
rabbit
m
分裂溝はTCAのみだが弱い
Cleavage furrows can be weakly stained only by TCA fix.
HeLa
Rho2011-09-27 WBIF IHCIP
BD100
R73920
m
m

HeLa
Rho (N-term)2011-09-27 WBIF IHCIP
EPT100
1600-1
rabbit
m
TCA 染色は弱い
Weak staining
HeLa
RhoA2011-09-27 おそらく26C4と同クローン (probably clone 26C4)WBIF IHCIP
ARP100
01-4301M
mouse
m
TCA おそらく26C4ど同クローン
HeLa
RhoA2011-09-27 おそらく26C4と同クローン (probably clone 26C4)WBIF IHCIP
Cytoskeleton100
ARH01
m
m
TCAのみOK
Visualized only by TCA fix.
HeLa,MDCK II
RhoA2011-09-27 26C4WBIF IHCIP
Santa Cruz100
SC418
m
m
TCAのみOK
Visualized only by TCA fix.
HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのラット組織
RhoA (67B9)2011-09-27 WBIF IHCIP
Cell Signaling100
2117
rabbit
m
TCA
HeLa
RhoA/B/C2011-09-27 WBIF IHCIP
Santa Cruz100
sc-179
rabbit
p
TCAのみOK
Visualized only by TCA fix.
HeLa, MECK II, ほとんどのマウス、ラット組織
RhoA/C2011-09-27 Lulu51WBIF IHCIP
Yonemura50-100
rat
m

HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのマウス組織
RhoABC2011-09-27 clone 55WBIF IHCIP
Upstate100
05-778
mouse
m
TCA
HeLa
Rhodopsin2011-11-26 4C5WBIF IHCIP
Developmental study hybridoma bank Cx15000x2000
4%PFA +0.1%Triton
mouse
IgG

Drosophila eye

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
RIPK1 (human/mouse)2016-08-10 WBIF IHCIP
BD Biosciences1/500
610459
Mouse
IgG2a

MEF, HT29
RIPK3 (mouse)2016-05-16 WBIF IHCIP
Prosci1/1000
2283
Rabbit

MEF
Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Rock-12011-09-27 WBIF IHCIP
Santa Cruz100
sc-6056
goat
p
1%PFA, TCA, MeOH
DLD?1
ROCK-22011-09-27 WBIF IHCIP
Santa Cruz100
sc-1851
goat
p
4%PFA, 1%PFA, MeOH, TCA
DLD-1, EpH4
ROCK12011-09-27 clone EP786YWBIF IHCIP
EPT100
1761-1
rabbit
m
分裂溝の染色はTCAのみ
Cleavage furrows can be stained only by TCA fix.
HeLa
ROCK12013-02-01 EPR638YWBIF IHCIP
Epitomicsx500x200
1953-1
4% PFA
rabbit (mono)

HeLa
Rubicon2011-09-27 1H6WBIF IHCIP
MBL1:1000
M170-3
mouse
m
WBでhumanのみ検出可能
Rubicon2011-09-27 WBIF IHCIP
MBL1:1000
PD027
rabbit
p
WBでhumanのみ検出可能
RUFY12011-08-29 3A5WBIF IHCIP
Abnovaダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a

Hela細胞
sheep Red Blood Cells2011-08-10 SRBCWBIF IHCIP
ICTx100
A6840
3%PFA (in PBS)
rabbit

羊赤血球

【S】

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
bovine synaptophysin2011-08-10 WBIF IHCIP
Chemicon
MAB5258
mouse
m

human CDCrel-12011-08-10 septin 5WBIF IHCIP
IBLx100
18921
3%PFA (in PBS), methanol
rabbit

マウス小腸
human septin 72011-08-10 CWBIF IHCIP
IBLx100
18991
3%PFA (in PBS), methanol
rabbit

マウス小腸
human SNAP-252011-08-12 WBIF IHCIP
Chemicon
AB5871P
goat
p
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
human SNAP-252011-08-10 WBIF IHCIP
Chemicon
AB5871P
goat
p

human somatostatin2011-08-10 WBIF IHCIP
Biogenesisx100
83300009
3%PFA (in PBS)
rat
m

マウス膵臓
human synaptobrevin2011-08-10 VAMPWBIF IHCIP
Chemicon
MAB335
mouse
m

human synaptobrevin2011-08-10 VAMPWBIF IHCIP
Chemicon
MAB335
mouse
m

mouse SGLT-12011-08-10 M-19WBIF IHCIP
Santa Cruzx100
sc20582
3%PFA (in PBS)
goat
p

マウス小腸
p70 S6 Kinase2017-08-02 49D7WBIF IHCIP
Cell Signaling Technology1/1000
2708
Rabbit

HEK293, HeLa
phospho-p70 S6K (T389)2017-08-02 108D2WBIF IHCIP
Cell Signaling Technology1/1000
9234
Rabbit

HEK293, HeLa
Phospho-S6 Ribosomal Protein (Ser235/236)2013-09-12 D57.2.2EWBIF IHCIP
Cell Signaling Technologyx1000
#4858
Rabbit monoclonal
IgG

HeLa
phospho-Smad1/5/82011-09-22 WBIF IHCIP
Cell Signaling Techx250x50
9511
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly?

mouse E6.5 embryo. MEF
phospho-Smad2/32011-09-22 WBIF IHCIP
Cell Signaling Techx250x50
3108
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab
incubate w/ Phosphatase inhibitors
mouse E6.5 embryo. MEF
S100beta2012-12-12 EP1576YWBIF IHCIP
abcamx200
ab52642
4%PFA (in PBS)
rabbit mo
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
mature astrocyte
mouse brain
S2P2011-08-29 WBIF IHCIP
Cell Signaling
rabbit
Poly

Hela細胞
S2P2011-08-29 WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
S2P2011-08-29 WBIF IHCIP
SIGMA
HPA005494
rabbit
Poly

Hela細胞
S6 Ribosomal Protein2013-09-12 54D2WBIF IHCIP
Cell Signaling Technologyx1000
#2317
mouse
IgG1

HeLa
Scrib2011-09-27 WBIF IHCIP
Santa Cruz100
sc-11049
goat
p
4%PFA, 1%PFA, TCA
DLD?1
Scrib2013-02-01 7C6.D10WBIF IHCIP
Milliporex100
MAB1820
3.7% FA
mouse
IgG1

MDCK2
sec152011-08-29 WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
sec15B2011-08-29 WBIF IHCIP
Santa Cruzx200
goat
Poly
IFは試してない,WBも微妙
Not tested for IF. Not good staining for WB.
Hela細胞
sec31A2011-08-29 WBIF IHCIP
BD Transductionx200?
mouse,IgG1
【PFA+Triton】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono

Hela細胞
Sec62011-08-14 WBIF IHCIP
Stressgen
VAM-SV021
mouse
m

Sec82011-08-14 WBIF IHCIP
BD transduction
610658
mouse
m

SEL1L2018-07-26 WBIF IHCIP
Sigma1/10001-3ug
S3699
Rabbit

HCT116
SEPT62011-08-14 WBIF IHCIP
Atlasx100
HPA005665
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
Serotonin, 5-HT2011-11-26 S5545WBIF IHCIP
sigmax200
S5545
4%PFA +0.1%Triton
rabbit

Drosophila nervous system
Serotonin, 5-HT2019-09-10 WBIF IHCIP
ImmunoStarx500-1000
20080
Parrafin section
Rabbit
IgG
Antigen retrieved with Immunosaver (Nisshin EM)
マウス小腸
SGLT2013-07-04 WBIF IHCIP
santa cruzx100
sc-20581
3%PFA (in PBS)
goat
0.05%saponin

Caco2

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
SGLT2013-12-04 WBIF IHCIP
santa cruzx100
sc-20581
3%PFA (in PBS)
goat
0.05%saponin

MDCK

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
SGLT-12011-08-14 WBIF IHCIP
CHEMICONx100
AB1352
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
sidestep2011-11-26 9B8WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo
slit2011-11-26 C55S.6DWBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo
smoothened2011-11-26 20C6WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
SNAP-232011-08-10 WBIF IHCIP
Synaptic Syx100
111202
3%PFA (in PBS)
rabbit
p
自家製より弱いのとspecificity低い
Weaker and lower specificity than homemade antibody
マウス脳
SNAP232011-08-10
About Product
PolyclonalWBIF IHCIP
Abcam
ab50357
rabbit
IgG

SNAP23A2011-08-14 WBIF IHCIP
Stressgenx100
VAP-SV013
rabbit
p

SNX12011-08-29 Clone 51WBIF IHCIP
BD Transductionx100-
mouse
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1

Hela細胞
Sodium Potassium ATPase2011-09-27
About Product
MonoclonalWBIF IHCIP
Abcam200-500
ab7671
mouse
IgG1
4%PFA, 1%PFA, TCA
DLD?1
somatostatin2012-12-12 WBIF IHCIP
Chemiconx100
AB5494
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
a type of interneuron
mouse brain
Sox12012-12-12 WBIF IHCIP
Santa Cruzx100
sc-17318
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
Sox22012-12-12 WBIF IHCIP
Chemiconx100
AB5603
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
Sox22012-12-12 WBIF IHCIP
Santa Cruzx100
sc-17320
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
Sox92011-08-10 WBIF IHCIP
millipore
ab5535
3%PFA (in PBS)
rabbit
p

マウス小腸
SP-C2011-08-10 C-19WBIF IHCIP
Santa Cruz
sc7705
goat
p

試してない?
Not used

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
SP-D2015-01-13 WBIF IHCIP
Milliporex2500
AB3434
Mouse

mouse lung frozen section

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
spectrin2011-11-26 3A9WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Splicing Factor SC-352011-10-13 SC-35WBIF IHCIP
SIGMAX3000
S4045
mouse
PFA+Triton or MeOH
Mono, IgG1

HeLa細胞
Src2011-09-27 WBIF IHCIP
Santa Cruz100
sc-8056
mouse
m
Focal adhesion染まらず。おそらく使用不可
Not stain focal adhesion.
HeLa
SREBP22011-08-29 ID2WBIF IHCIP
MBLx500ダメ
mouse,IgG1
Mono

Hela細胞
SREBP22011-08-29 WBIF IHCIP
Caymanダメダメ
rabbit
Poly

Hela細胞
ssbp32011-08-10 mouseWBIF IHCIP
abnova
h00023648-m01
mouse
m

試してない?
Not used
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
STI-12012-11-13 WBIF IHCIP
BD Transductionx100
S65720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

Caco2細胞
STI-12012-11-13 WBIF IHCIP
BD Transductionx100
S65720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
STI1/Hop-p602012-01-25 WBIF IHCIP
BD Transductionx100
S65720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
STXBP62012-12-12 WBIF IHCIP
Sigma ATLASx200
HPA003552
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG granule cell
mouse brain
STxB2011-08-29 13C4WBIF IHCIP
ATCCx100
mouse
Mono

Hela細胞
Su(fu)2011-11-26 25H3WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
sucrase-isomaltase2012-04-04 HBB2/614/88 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG1
culture sup
Caco2
JCB 101, 838-851, 1985
sucrase-isomaltase2012-04-04 HBB2/219/20 monoclonalWBIF IHCIP
Developmental Studies Hybridoma Bankx5
3%PFA in PBS
mouse
0.05% saponin
IgG2b
culture sup
Caco2
JCB 101, 838-851, 1985
SUN12011-10-13
About Product
PolyclonalWBIF IHCIP
AbcamX 500X200
ab74758
rabbit
PFA+Triton or MeOH
IgG

HeLa細胞
SUN22011-10-13
About Product
PolyclonalWBIF IHCIP
AbcamX500~1000X200
ab65447
rabbit
PFA+Triton or MeOH
IgG

HeLa細胞
SV22011-08-12 WBIF IHCIP
DSHB
SV2
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
SV22011-08-10 WBIF IHCIP
DSHB
SV2
mouse

SV22012-08-03 WBIF IHCIP
DSHBx100
SV2
3%PFA in PBS
mouse
0.05% saponin

PC12
SYBL12011-08-10
About Product
MonoclonalWBIF IHCIP
Abcamx100
ab36195
mouse
IgG1
WBで染まるが、KOでもバンドが出るため、VAMP7以外のものを認識しているようだ
Can detect proteins by WB even in VAMP7 knockout mice, so some other proteins may be detected.
synapsin I2012-12-12 WBIF IHCIP
abcamx500
ab8
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
synapse
mouse brain

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptobrevin 2 (VAMP2)2016-08-10 69.1WBIF IHCIP
Synaptic systems1/10001/500
104211
4% PFA
Mouse
IgG

primary neuron, PC12

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptobrevin/VAMP 22011-08-10 WBIF IHCIP
Synaptic Sy
104211
mouse
m

synaptobrevin/VAMP 22011-08-12 WBIF IHCIP
Synaptic Sy
104211
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
synaptobrevin/VAMP 22011-08-10 WBIF IHCIP
Synaptic Syx1000
104211
3%PFA (in PBS)
mouse
m

マウス
Synaptophysin2011-10-03 WBIF IHCIP
Progenx1000x2500
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Mono,

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
synaptophysin2012-12-12 WBIF IHCIP
Chemiconx100
MAB368
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
synapse
mouse brain

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptophysin 12016-10-18 7.2WBIF IHCIP
Synaptic systems1/10001/500
101011
4% PFA
Mouse
IgG

primary neuron, PC12
synaptophysin/p382014-05-09 WBIF IHCIP
Millipore (Roche)x1000
MAB5258
3%PFA (in phosphate buffer)
mouse
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum
synaptotagmin 12016-10-18 41.1WBIF IHCIP
Synaptic systems1/10001/500
105011
4% PFA
Mouse
IgG

primary neuron, PC12

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
syntaxin2011-11-26 8C3WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse
IgG

Drosophila embryo, discs, nervous system

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
syntaxin 12016-09-08 78.2WBIF IHCIP
Synaptic systems1/10001/500
110011
4% PFA
Mouse
IgG

primary neuron, PC12
syntaxin 12/132016-08-10 WBIF IHCIP
Synaptic systems1/10001/500
110133
4% PFA
Rabbit
IgG

HeLa, PC12

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Syntaxin 162011-08-29 WBIF IHCIP
SySyx1000-
rabbit
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
syntaxin 32011-08-10 WBIF IHCIP
Synaptic Syx100
110033
3%PFA (in PBS), methanol
rabbit
p

WB, マウス小腸共
syntaxin 32011-08-10 WBIF IHCIP
GeneTexx100
GTX24113
3%PFA (in PBS), methanol
rabbit
p

WB, マウス小腸共
syntaxin 32011-08-10 WBIF IHCIP
Chemiconx100
AB5448
3%PFA (in PBS), methanol
rabbit
p

WB, マウス小腸共
Syntaxin 32011-08-10 WBIF IHCIP
Sigmax100
S5547
3%PFA (in PBS), methanol
rabbit

WB, マウス小腸共
Syntaxin 42011-08-10 WBIF IHCIP
Sigmax100
S9924
3%PFA (in PBS), methanol
rabbit

WB, マウス小腸共
Syntaxin 52011-08-29 WBIF IHCIP
SYSYx200-
rabbit
Poly
もう少し希釈倍率を上げても良いと思います(IF)
Can be diluted further for IF
Hela細胞
Syntaxin 62011-08-29 610635WBIF IHCIP
BD Transductionx100
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
Syntaxin 62011-08-29 WBIF IHCIP
SySyx200
rabbit
Poly
BDのものよりWB良い。
Better Ab for WB than BD Ab
Hela細胞

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
syntaxin 62016-09-08 WBIF IHCIP
Synaptic systems1/10001/500
110062
4% PFA
Rabbit
IgG

HeLa, PC12

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Syntaxin 82011-08-29 WBIF IHCIP
SySyx200
rabbit
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
Syntaxin12011-08-14 HPC-1WBIF IHCIP
MBL
K0117-3
mouse
m

syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma5000
S0664
mouse

pancreas lysate
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma100
S0664
3%PFA (in PBS)
mouse

mouse primary neuron, neural progenitor cell, pancreas
Syntaxin12/132011-08-29 WBIF IHCIP
SySy
rabbit
【PFA+Triton】:×【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Poly

Hela細胞
Syntaxin32011-08-14 WBIF IHCIP
Atlas
HPA002191
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
syntaxin32013-07-04 WBIF IHCIP
sigmax1000
s5567
rabbit
nonspecific band at ca.30k even in KO tissue
mouse brain, intestine
syntaxin32013-07-04 WBIF IHCIP
chemiconx1000
AB5448
rabbit
nonspecific band at ca.30k even in KO tissue
mouse brain, intestine
syntaxin32013-07-04 WBIF IHCIP
gene texx1000
GTX24113
rabbit
nonspecific band at ca.30k even in KO tissue
mouse brain, intestine
syntaxin32013-07-04 WBIF IHCIP
synaptic systemsx1000
rabbit
no staining
mouse brain, intestine
syntaxin32013-07-04 WBIF IHCIP
atlas antibodies (sigma)x1000
HPA002191
rabbit
no staining
mouse brain, intestine

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
syntaxin32013-12-04 WBIF IHCIP
gene texx100
GTX24113
3%PFA (in PBS)
rabbit
0.05%saponin

MDCK
Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Syntaxin42011-08-14 WBIF IHCIP
BD transduction
610439
mouse
m

Syntaxin42012-11-13 49WBIF IHCIP
BD Transductionx1000
610439
mouse
IgG1

マウス膵臓ライセート
Syntaxin62012-01-25 WBIF IHCIP
BD Transductionx100
S55420
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
syntaxin62012-05-21 WBIF IHCIP
BD Transduction Labx100
S55420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
syntaxin62012-05-21 WBIF IHCIP
BD Transduction Labx100
S55420
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK

【T】

【t】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Acetylated Tubulin2011-08-10 WBIF IHCIP
SIGMAx100
T7451
3%PFA (in PBS)
mouse
m

マウス組織細胞など
Acetylated tubulin2011-10-03 6-11B-1WBIF IHCIP
sigma×50000
T7451
mouse
【Formalin in PBS+0.05%Triton】:○
Mono

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
AcetylTubulin2011-08-29 WBIF IHCIP
SIGMAx200-
mouse
【PFA+Triton】:◎,◎(CanGetSignalA (from TOYOBO Inc.)),【TCA】:◎,◎(CanGetSignalA (from TOYOBO Inc.))【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
Mono、IgG2b

Hela細胞
alpha-tubulin2018-07-12 WBIF IHCIP
MBL1/2000
PM054
rabbit

Drosophila pupa
b-Tubulin2011-08-29 KMX-1WBIF IHCIP
Chemiconx1000
mouse
【PFA+Triton】:○,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG2b

Hela細胞
beta 3-tubulin2011-10-03 Tuj1WBIF IHCIP
CovanceX2000
MMS-435P
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,
Mono, IgG2a

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
beta-tubulin2011-09-17 9F3WBIF IHCIP
Cell Signalingx1000
#2128
rabbit
Mono

Hela細胞
beta-tubulin2011-09-28 9F3WBIF IHCIP
Cell Signalingx1000
#2128
rabbit
Mono

HeLa細胞
Biotin mouse CD712011-08-10 TFRWBIF IHCIP
BD Pharmingenx100
557416
3%PFA (in PBS), methanol
rat
m

マウス小腸
Biotin mouse CD712011-08-10 TFRWBIF IHCIP
BD Pharmingenx100
557416
3%PFA (in PBS), methanol
rat
m

マウス小腸
bovine tau-12011-08-10 WBIF IHCIP
Chemiconx100
MAB3420
3%PFA (in PBS)
mouse
m

マウス脳
CD712011-08-10 TFRWBIF IHCIP
Zymedx100
77103
3%PFA (in PBS), methanol

マウス小腸
CD712011-08-10 R17217 TFRWBIF IHCIP
Santa Cruzx100
sc52504
3%PFA (in PBS), methanol
rat
m

マウス小腸
CD712011-08-10 TFRWBIF IHCIP
Zymedx100
77103
3%PFA (in PBS), methanol

マウス小腸
CD712011-08-10 R17217 TFRWBIF IHCIP
Santa Cruzx100
sc52504
3%PFA (in PBS), methanol
rat
m

マウス小腸
Detyrosinated (Glu-)tublin2011-09-27 WBIF IHCIP
chemicon100
AB3201
rabbit
p
不明瞭
Staining not evident
HeLa
FITC mouse TCR-b chain2011-08-10 WBIF IHCIP
BioLegend
109205
hamster

g-tubulin2011-08-29 GTU-88WBIF IHCIP
SIGMAx1000
T6557
mouse
【PFA+Triton】:○,【IS】:○,【TCA】:◎【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
mono, IgG1
セミカルバジドでquencing,またはCanGetSignalA (from TOYOBO Inc.)でみえる
Quenching by Semicarbazide is needed. Or use CanGetSignalA (from TOYOBO Inc.)
Hela細胞
gamma-tubulin2011-10-06 H-183WBIF IHCIP
SantaCruzx400
sc-10732
Rabbit
Methanol: ○
polyclonal

HeLa
Glu-alpha-tublin2011-09-27 WBIF IHCIP
SS100
302 011
mouse
m
不明瞭
Staining not evident
HeLa
GoatTCP-1 ε2011-08-10 C-15WBIF IHCIP
Santa Cruz
sc13886
goat
p

GoatαTCP-1ζ2011-08-10 A-15WBIF IHCIP
Santa Cruz
sc13896
goat
p

human tamm-horsfall glycoprotein2011-08-10 WBIF IHCIP
Biocedical Tx100
BT590
3%PFA (in PBS)
rabbit

マウス腎臓
human Transferrin Receptor2011-08-12 WBIF IHCIP
Zymed
136800
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human Transferrin Receptor2011-08-10 WBIF IHCIP
Zymedx100
136800
3%PFA (in PBS), methanol
mouse

マウス小腸
human Transferrin Receptor2011-08-10 WBIF IHCIP
Zymedx100
136800
3%PFA (in PBS), methanol
mouse
MDCKは染まる 3T3は染まらない
Stains MDCK, but not 3T3.
マウス小腸
human Transferrin Receptor2012-08-03 WBIF IHCIP
Zymedx100
136800
3%PFA in PBS
mouse
0.05% saponin

PC12
mouseαNeuronal classαTubulin2011-08-10 WBIF IHCIP
Covancex100
mms-435p
3%PFA (in PBS)
mouse
m

マウス脳
mouseαTyrosine Hydroxylase2011-08-10 WBIF IHCIP
milliporex100
MAB318
3%PFA (in PBS)
mouse
m

マウス脳
phospho-Tyr2011-10-17 WBIF IHCIP
Cell SignalingX500
#9411
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono, IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
rabbitαTbr12011-08-10 WBIF IHCIP
CHEMICONx100
ab9616
3%PFA (in PBS)
rabbit

マウス脳
rat TGN382011-08-12 WBIF IHCIP
Serotec
AHP499G
sheep
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
rat TGN382011-08-10 WBIF IHCIP
Serotecx100
AHP499G
3%PFA (in PBS), methanol
sheep
メタノール固定で染まるが形がよくない
Visualized by methanol fixation, but the TGN is not well preserved.
マウス小腸
rat TGN382012-08-03 WBIF IHCIP
Serotecx100
AHP499G
3%PFA in PBS
sheep
0.05% saponin

PC12
rat thyrotropin2011-08-10 TSHWBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACRT2901RBP86
3%PFA (in PBS)
rabbit

マウス下垂体
T(ヒト赤血球)2011-11-26 HB-T1WBIF IHCIP
DAKO
M0898
4%PFA +0.1%Triton
mouse IgM
IgM

Drosophila embryo, discs, nervous system
T72011-08-29 69522-3WBIF IHCIP
novagenx10000
mouse
Mono, IgG2b

Hela細胞
Tau-12011-10-03 PC1C6WBIF IHCIP
Chemiconx500
MAB3420
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,
Mono, IgG2a

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Tau12011-08-14 WBIF IHCIP
CHEMICONx1000x100
MAB3420
3%PFA (in PBS)
mouse
m

マウス脳
TBP(TFIID)2011-08-29 1TBP18WBIF IHCIP
SantaCruzx200
mouse
Mono

Hela細胞

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Tbr12014-05-09 WBIF IHCIP
Milliporex500
AB9616
3%PFA (in phosphate buffer)
rabbit
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum
Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
TCF-42011-08-10 H-125WBIF IHCIP
Santa Cruz
sc13027
rabbit
p

TCF42011-08-14 WBIF IHCIP
Cell Signaling
2565S
rabbit
m

TFE32011-09-17
About Product
WBIF IHCIP
Abcam
ab24797
rabbit

Hela細胞
TFE32011-09-17
About Product
PolyclonalWBIF IHCIP
Abcam
ab70008
rabbit
IgG

Hela細胞
TFE32011-09-17
About Product
PolyclonalWBIF IHCIP
Abcamx1000x100
ab93808
rabbit
IgG

Hela細胞
TFE32011-09-28
About Product
WBIF IHCIP
Abcamx250
ab24797
rabbit

HeLa細胞
TFE32011-09-28
About Product
PolyclonalWBIF IHCIP
Abcamx1000x100
ab93808
rabbit
IgG

HeLa細胞
TFE32011-09-28
About Product
PolyclonalWBIF IHCIP
Abcamx250
ab70008
rabbit
IgG

HeLa細胞
TFEB2011-09-17 WBIF IHCIP

Hela細胞
TFEB2011-09-28
About Product
PolyclonalWBIF IHCIP
Abcamx250
ab2636
goat
IgG

HeLa細胞
Tfn (HRP)2011-08-29
About Product
MonoclonalWBIF IHCIP
Abcam
ab74415
mouse
IgG1
x24000 For ELISA
Hela細胞
TfnR2011-08-29 H68.4WBIF IHCIP
Zymedx1000-x5000-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1
Human OK, MDCKII OK, OverexpressionしているArfraptin2を認識する。
Recognize overexpressed Arfaptin2.
Hela細胞
TfnR(CD71)2011-08-29 CD71WBIF IHCIP
SIGMAx200-
C2063
mouse
【PFA+Triton】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1
Lumen側を認識する抗体
Ab recognize luminal part.
Hela細胞
TfR2011-09-27 WBIF IHCIP
BD Biosciences1:10001:100
612125
mouse
m
human専用
HeLa, A549, MCF7他
TfR2011-09-27 H68.4WBIF IHCIP
Zymed1:10001:100
13-6800
mouse
m

HeLa, A549, MCF7他
TGN462011-08-29 WBIF IHCIP
Cosmo biox200
sheep
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Poly
2ndはDonkey anti-sheep Cy3を使用、MDCKIIダメ
Use Cy3 labeled donkey anti-sheep as 2nd Ab. Not suitable for MDCKII.
Hela細胞
Thioredoxin2011-08-29 WBIF IHCIP
SIGMAx5000
T0803
rabbit
Poly

Hela細胞
TIP472011-08-29 WBIF IHCIP
Progenx1000x200
guinea pig
【PFA+Saponin】:○,【PFA_70%EtOH】:○,【PFA_digitonin】:○【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS【digitonin条件】50ug/ml digitonin /PBS
Poly

Hela細胞
TLR42011-08-29 HTA125WBIF IHCIP
IMGENEXx500x200
mouse
Mono, IgG2a

Hela細胞
Tn(ヒツジのムチン sheep mucin)2011-11-26 HB-Tn1WBIF IHCIP
DAKO
M0896
4%PFA +0.1%Triton
mouse IgM
IgM

Drosophila embryo, discs, nervous system
Tollip2011-08-29 Kimmy-2WBIF IHCIP
ALEXISx500x200
mouse
Mono, IgG1

Hela細胞
Tpr2011-10-13 203-37WBIF IHCIP
Matri-TectX1000X500
M1200
mouse
MeOH
Mono, IgG1

HeLa細胞, TIG-1細胞
trachea2011-11-26 2A12WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse IgM
IgM

Drosophila trachea
tsg1012011-08-29 4A10WBIF IHCIP
GeneTexx1000x200
mouse
【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【saponin条件】0.05% saponin /PBS
Mono, IgG1
IF:saponin permeabilizeでendo Tsg101見える
Endogenous Tsg101 can be recognized by saponin perm.
Hela細胞
tsg101N2011-08-29 AP2155aWBIF IHCIP
ABG
Rabbit
Poly

Hela細胞
tubulin2011-10-06 WBIF IHCIP
Cytoskeletonx400
ATN02
Sheep
4%PFA+0.1%Saponin :○
polyclonal

HeLa
tubulin2011-10-28 WBIF IHCIP
Cytoskeletonx400
ATN02
4%PFA+0.1%Saponin :○
Sheep
polyclonal

HeLa
Tubulin2011-09-27 WBIF IHCIP
Sigma100
T3526
rabbit
p
染まらず
Not stained
MDCK II
tubulin2011-11-26 T5192WBIF IHCIP
sigmax1000
T5192
rabbit

Drosophila embryo, discs, nervous system
tubulin2011-11-26 DM1AWBIF IHCIP
Sigmax1000
T9026
mouse

Drosophila embryo, discs, nervous system
tubulin(β)2011-11-26 WBIF IHCIP
Developmental study hybridoma bankx100
mouse

Drosophila embryo, discs, nervous system
tubulin(γ)2011-11-26 GTU88WBIF IHCIP
Sigmax3000
T6557
mouse

Drosophila embryo, discs, nervous system
Tubulin, Acetylated2011-09-27 WBIF IHCIP
Sigma100
T6793
mouse
m
4%PFA>MeOH, TCA
MDCK II
Tubulin, alpha2011-08-29
About Product
MonoclonalWBIF IHCIP
Abcam
ab6160
rat
【TCA】:○【TCA条件】TCA 10%/H2O on ice 15min
IgG2a
凍るためGlycerol添加(final 66.6%、a-Tubulin 50ul + glycerol 25 ul)
To prevent freezing, add glycerol (final 66.6%、a-Tubulin 50ul + glycerol 25 ul)
Hela細胞
Tubulin, alpha2011-09-29
About Product
MonoclonalWBIF IHCIP
Abcamx5,000~x50~100
ab6160
rat
IF: -20℃ methanol or acetone:methanol=1:1
IgG2a

HeLa細胞
Tubulin, Tyrosine2011-10-03 TUB-1A2WBIF IHCIP
Sigma×1500
T9028
mouse
【Formalin in PBS+0.05%Triton】:○
Mono,IgG3

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Tyr-alpha-tub2011-09-27 WBIF IHCIP
SS100
302-111
mouse
m
不明瞭
Staining not evident
HeLa
Tyrosine hydroxylase (TH)2012-12-12 WBIF IHCIP
Chemiconx500
AB152
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
OB periglomerular neuron
mouse brain
α-Tubulin2011-08-10 clone:DM1AWBIF IHCIP
Sigmax100
T9026
3%PFA (in PBS)
mouse
m

マウス脳など
α-Tubulin2011-09-29 DM1AWBIF IHCIP
SIGMAx10,000~x50~100
T9026
mouse
IF: -20℃ methanol or acetone:methanol=1:1
mono, IgG1

HeLa細胞
α-tubulin2011-10-03 WBIF IHCIP
Cell Signaling×250
2144
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
α-Tubulin2011-09-27 B-5-1-2WBIF IHCIP
SIGMA1:2000~1:50001:1000
T6074
mouse
m
IF: 固定、permeabilizeは37℃で行う
MEF, HeLa, A549, MCF7他
α-Tubulin2011-10-17 DM1AWBIF IHCIP
SigmaX1000X500
T6199
mouse
【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
Mono
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
α-Tubulin2011-09-27 clone DM1AWBIF IHCIP
Sigma200-500
T9026
mouse
m
4%PFA, MeOH, TCA
試した限り全ての培養細胞、組織
α-Tubulin−FITC2011-09-29 DM1AWBIF IHCIP
SIGMA-x50~100
F2168
mouse
IF: -20℃ methanol or acetone:methanol=1:1
mono, IgG1

HeLa細胞
β-Tubulin2011-08-10 clone:TUB2.1WBIF IHCIP
Sigmax100
T4026
3%PFA (in PBS)
mouse
m

マウス脳など
γ-Tubulin2011-08-10 WBIF IHCIP
SIGMAx100
T5192
3%PFA (in PBS), methanol
rabbit
p
メタノール固定でOK
Methanol fixation alone is also suitable.
マウスMEF
γ-Tubulin2011-10-03 GTU-88WBIF IHCIP
sigmax1000x500
T6557
mouse
【Formalin in PBS+0.05%Triton】:○
Mono, IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
γ-Tubulin2011-10-17 WBIF IHCIP
SigmaX500
T5192
rabbit
【MeOH -20℃ 5min】:○
【3.7% formalin solution 37℃ 5min+MeOH -20℃ 15min】:○,
【3.7% formalin solution+Triton】:×
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
HeLa細胞
γ-Tubulin2011-10-13 GTU-88WBIF IHCIP
SIGMAX500
T6557
mouse
Mono, IgG1

HeLa細胞